Table of Contents
Advertisement
+
CombiFlash® Rf
User Manual
load cartridge, and loaded the cartridge on the
system. You must also specify the cartridge type and
size (Column plan subscribers only). The system will
automatically proceed until the end of the
programmed run length.
• Liquid – Select this option if you have prepared a
liquid sample and plan to manually inject it into the
injection port after column equilibration.
• None (on column) – Select this option if you have
preloaded the sample on the column. The system will
skip column equilibration so that the sample will not
be flushed from the column before the run.
Loading types for the Rf150 system are:
• Equilibration – Select this option if you intend to
equilibrate the column before introducing the liquid
or solid sample.
• No Equilibration – Select this option to skip column
equilibration.
5.Review or update the Start Rack and Start Tube.
6.Review the Minimum Run Requirements. The system will
report the estimated solvent volumes, expected waste,
collection tube usage, etc. You can use this information to
verify that there will be enough solvent to complete the
run, the waste volume does not exceed the collection
container's capacity, and whether or not more tubes will be
required during the run.
Note: The Rf+ systems, including those equipped with
4x estimate the solvent volumes by monitoring the
solvent level in the container and the known usage rate.
The system continues to refine this estimate during
operation. See also: A
[
-
ON
LINE
Note: If the waste level sensing tube is inserted
correctly into the waste container, the system
automatically will suspend operation before an overflow
condition might exist. To prevent the run from being
suspended before completing the run, ensure that the
container will hold the expected waste volume. Waste
level sensing is not available on the Rf150 system.
6-44
BOUT
.
]
S
L
OLVENT
EVEL
D
ETECTION
Advertisement
Table of Contents
