Starting The Separation - Teledyne CombiFlash Rf User Manual

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CombiFlash® Rf User Manual
requires an injection syringe to use a 22 gauge blunt needle
for injection. he adapter can be obtained as Teledyne Isco
PN/29-9125-060.

5.5.3 Starting the Separation

1. On the main screen, press the RediSep drop down.
2. Select the Prep HPLC Column from the drop down menu.
This loads the HPLC column that was selected as "Default"
in the configuration screen. The screen color scheme will
change to green signifying the HPLC portion of the
instrument will be used.
• If this is the desired column, simply press the P
button to continue.
• If a different HPLC column is preferred, select the
RediSep drop down again, and select the desired
HPLC column, or select "Flash Column" to return to
flash operation.
3. The sample loading technique must be selected. L
assumes that your sample is ready for loading. If that is
correct, inject the sample into the HPLC inject port.
Remember that the sample volume should not exceed ½ of
the loop volume.
4. Then press the sample loaded button to start the
separation.
• If L
E
equilibration. This allows extra time to prepare the
sample while the column is being equilibrated. At the
completion of the equilibration phase, the system will
stop, prompting injection of the sample. After
acknowledgement of the sample injection the run will
continue.
• Operation now proceeds as described for a flash
separation. At the end of the separation, the flash
valve wash and column air purge are not performed.
5-18
A E
OAD FTER
button can be pressed to begin the
QUILIBRATION
is selected, the S
QUILIBRATION
LAY
N
OAD OW
TART