Prep Hplc - Teledyne ACCQPrep HP125 Installation And Operation Manual

3.4 Prep HPLC

reviewing a previous separation, this information isn't available on
the screen, but many times, the profile of the trace is adequate for
troubleshooting.
Show Absorbance During Equilibration – Shows absorbance
of the system during equilibration to verify baseline stability.
Pressure Units – You can choose between PSI and Bar.
Enable Automatic Peak Hold – Allows the system to go into an
isocratic condition whenever a peak is detected to get the current
compound off while not rushing the other compounds off the
column. The length of the separation is increased by the amount
of time the system is in the isocratic hold condition. At the end of
the detected peak, the system will automatically exit the hold
condition.
Note
If a PurIon mass spectrometer detector is attached, you can
set the D MS G
EFAULT
trol denotes the starting mass displayed in the mass spectrum
graph. The X-AXIS END control denotes the ending mass dis-
played in the mass spectrum graph. In either case, the entire
mass spectrum (10 Da through 1200 (2000 Da PURION L
only) is always acquired and stored and can be viewed later
opening the format graph button on the results screen.
Prep HPLC can be found under: T
HPLC
Loop Volume – Ensures that the samples entered do not exceed
the loop volume amount per injection. A warning will display if
half of the loop volume is exceeded, but it will not limit the
injection amount that can be programmed. This warning can be
ignored.
Column Position – This allows you to define a column(s) used on
the instrument. If you do not have the Column Selector Valve
Module (CSV-4) you can define multiple columns and select
which column is currently connected. If you have the CSV-4
installed, you can configure which columns are installed. The
name of the column used for the separation is recorded on the
report. This name can be descriptive for the column, or identify it
exactly using the column serial number.
Probe Type – This defaults to the standard, "Teflon" probe sup-
plied with the instrument. It allows selection of an optional
"Steel" needle available for piercing vials with an aluminum foil
cover to prevent evaporation. This optional probe is NOT able to
pierce a silicone septa. The steel probe has a slightly different
internal volume that requires a different compensation volume.
Bracketed Sample – This allows the sample injection to be
"bracketed" with air and a user supplied solvent to address
potential solubility issues when injected. If this button is acti-
vated, each separation defaults to using a bracketed sample
injection protocol that can still be overridden for individual
samples. Further details can be found in Section 4.3.5.
ACCQPrep HP125
Section 3 Configuration
settings The X-AXIS START con-
RAPH
> C
OOLS ONFIGURATION
> P
REP
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