System Verification - Teledyne CombiFlash Rf User Manual

Section 2 Preparation: Rf + , Rf + Lumen, Rf+ PurIon, and EZ Prep Systems
beneficial to flush all of the fluid lines with the desired solvent
to remove any isopropanol left from production testing. This
can be done with the manual control feature (Section 6.3.9).
Note
Use Auto Prime for quick solvent changes of similar polarity by
pumping a fixed amount of solvent through the waste fluid path at
100 mL/min. For more advanced priming functions such as
varying the flow rates, fluid paths, etc., or to purge the system with
air before changing between normal and reversed phase solvent
systems, use the Tools>Manual Control option.

2.15 System Verification

It is recommended that the system operation be verified.
Verification of the EZ Prep system is also accomplished by
performing the defined normal phase procedure below. This
tests all EZ Prep system functions also. To do so, use a
pre-packaged CombiFlash Rf test kit:
• Part number 60-5237-050
Test kit containing five of the 5 gram size solid load
cartridges pre-filled with 0.5 grams of test sample
NPHE, and five 4 gram RediSep Rf silica gel columns
• Part number 60-5237-051
Test kit containing four of the 25 gram size solid load
cartridges pre-filled with 0.5 grams of test sample
NPHE, and four 4 gram RediSep Rf silica gel columns
• Part number 60-5234-626
Test mixture supplied in the PurIon accessory kit,
containing 10 g of MSD test mix
• Part number 60-5234-315
Test mixture supplied with APCI probes, 5 mL
• Part number 60-5234-627
Test mixture supplied with ESI probes, 10 mL
Note
Verification of the EZ Prep is accomplished by verifying the flash
chromatography operation. All functionality of the EZ Prep is
verified by that process.
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