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CombiFlash® Rf
User Manual
• Liquid sample loaded onto column with air purge NOT
enabled:
If you suspend the run with the Stop button, remove the
column and install the Prime Tube in its place. Manually
purge the liquid lines by pressing Tools -> Manual Control
-> Air Purge Column.
• Next Tube — This button advances the fraction
collector to the next tube position. This allows you to
conveniently collect eluate of interest in a new tube.
Note
When you click the Next Tube button, the system immediately
marks the tube advance on the chromatogram. However, the
system delays the movement of the drop former over the tubes.
This delay accounts for the volume held in the tubing between the
detector and the drop former, sometimes called the "delay
volume." It is important to remember that the chromatogram
displays activity in real time while the fraction collector activity lags
behind. The duration of this delay will vary with the flow rate.
• Rewind — This button is active when the system has
completed the run, or if the run was stopped by clicking
the Stop button. The Rewind button returns you to the
Main window with the current method.
If you have stopped the run before its programmed run
length has elapsed, clicking the Rewind button changes the
current method to the new, shorter run length. This modi-
fied method is ready for the next run, or can be saved for
future runs.
• Fast Forward — Click this button to jump to the next
step of a run.
Once you have started a run, the system performs several
steps. The first step is to deliver solvents using the pro-
grammed gradient for the entire run length. When this step
is complete, the system will purge the column with air and
clean the injection valve. Clicking the Fast Forward button
will cause the system to skip any remaining time in the
current step and advance to the beginning of the next step.
Unlike the Rewind button, clicking the Fast Forward but-
ton to complete the run will not modify the current
method's run length setting.
5-12
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