Teledyne CombiFlash Rf User Manual page 169

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CombiFlash® Rf User Manual
As an example, consider a RediSep Rf 4 g column and a 3
column volumes setting. Before instructing you to inject the
sample, the system will pump 14.4 mL of solvent A through
the column and out the waste port.
Initial Waste & Time Windows The initial waste diverts a
user-determined line volume to the waste port until the eluant
is about to be collected. At the start of a run, the internal
solvent lines hold some solvent from the previous run. This
volume can be diverted to waste, along with the anticipated
volume of fluid that will pass through the column before a
compound of interest will elute.
Time Windows can limit the fraction collection to specific time
durations of the run. To use time windows enter a start time
and an end time. All times are relative to start of the run, just
following the sample injection. Then choose a peak collection
mode. If you select Peaks, the module will collect fluid only
when the time window is active and a peak is present. If you
select All, the module will collect all fluid during the time
window regardless of the peak state. Define up to three time
windows.
Air Purge You can program the system to purge the column
with air after a run. This feature allows you to remove
solvents from the column before it is discarded. Enter the
desired time to purge the column, in minutes. To disable the
post-run air purge, enter zero minutes.
Peak Collection There are three peak collection options: All,
Peaks, or None. The All option will collect peak and non-peak
fluid in tubes. The Peaks option will collect only peak fluid in
tubes and divert all other fluids to the waste port. The None
option will divert all fluids, peak and non-peak, to the waste
port. The None option is useful for developing custom methods
that perform a column wash, system cleaning, or similar
function where solvents and elute do not need to be collected.
When using the All or Peaks options, you can specify the
maximum Peak and Non-peak volumes to be collected in the
tubes. Be sure to enter volumes less than the maximum tube
capacity. Different peak and non-peak tube volumes can be
used to conserve tubes when using the All peak collection
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