Teledyne CombiFlash Rf User Manual page 170

mode by collecting large volumes of non-peak fluid while
creating more, smaller fraction volumes of fluids of interest.
The fraction collector will advance to the next tube whenever a
peak is detected. Also note that the system will advance to the
next tube if a new peak is detected before completing the last
one (sometimes called a double advance). Tubes may also
advance, when using multiple detectors, depending on
impurities or sensitivity to a given compound by each detector.
Keep in mind that the slope and threshold detection methods
use algorithms which optimize the peak detection. For
example, peak detection includes a hysteresis to reduce the
likelihood of multiple tube advances that may occur if there is
noise at the beginning or tail end of a peak.
Peak Detection This section of the window contains option
buttons to enable and disable various peak detection options.
When an option is enabled, the window also allows you to
modify the setting details for that peak detection option. All
enabled options will be displayed on the chromatogram. The
options and settings for the Rf+ systems, including those
equipped with 4x are described below.
Note
Note: You can select up to four peak detection options (excluding
the External option) on the Rf+ systems, including those equipped
with 4x. If using more than one option, such as 1 with  2, the
system considers a peak to be present when either option is true
(a logical OR).
• 1 and 2 (wavelength 1 and 2) — Enable these
options to use and configure primary and secondary
wavelength detection. When enabled, type or select the
peak detection wavelength in nanometers, then click the
Details button to configure additional settings described
below.
• Slope Based — Select this option to enable
Slope-based peak detection. When enabled, peaks will
be recorded if the slope algorithm indicates a peak
within the Peak Width setting.
• Peak Width — Select the average peak width
setting. Peak widths are measured at the baseline.
The slope detector will detect peak widths ranging
Section 6 User Help Reference
6-49