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• S
ETTING THE
6.4.1 Starting a run using default settings
After the CombiFlash Rf system has been set up and
automatically primed (See A
the system is ready to perform a run using the default
settings. These default settings are preset to the most common
configurations used by chemists, and have been optimized for
the use of RediSep Rf columns. The default settings will:
• collect all fluid in the fraction collector rack
• differentiate between UV (default is 254 nm) vs. non-UV
absorbing fluid and place the fluids into separate tubes
• cut UV absorbing peaks based on slope detection or level
threshold.
To start the run from the PeakTrak Main window:
1. Enter a sample name. If you choose not to, PeakTrak will
enter the date and time as the sample name when you start
the run.
2.Review the PeakTrak Main window settings. If you want to
change any settings, refer to the Editing a Method on-line
help topic.
3.Click the Play button.
4.Select the sample Loading Type from the list. The Loading
Type should support the sample injection technique you
intend to use (see S
Possible sample loading types for the Rf+ systems,
including those equipped with 4x are:
• Solid (Pause) – Select this option if you intend place
the sample into a solid sample load cartridge, but
have not yet prepared the sample. You must also
specify the cartridge type and size (Column plan
subscribers only). When you click the run button, the
system will perform a column equilibration and then
wait while you prepare the cartridge. After you have
placed the cartridge on the system, click OK to
continue with the run.
• Solid – Select this option if you have already
prepared the sample, placed it into a solid sample
V
L
APOR
IMIT [6.4.12]
UTO
I
AMPLE
NJECTION
Section 6 User Help Reference
P
/V
W
RIME
ALVE
M
ETHODS [
),
ASH [6.3.11]
).
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]
ON
LINE
6-43
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