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called an isocratic hold). Note that pausing the run
extends the run length. While in the Paused state, you
can resume the run by clicking the Play button, or stop
the run by clicking the Stop button. If you resume the
run, the system continues with the gradient curve at the
%B when the system was paused.
• Stop — The Stop button suspends the entire run.
Unlike the Paused state, the pump, peak detection, and
fraction collection will not operate. While stopped, you
can end the run by clicking the Rewind button, resume
the run by clicking the Play button, or skip the rest of
the run sequence by clicking the Fast Forward button.
+
(All Rf
Systems)— When the Stop button is pressed, solvent
remains in the tubing from the injection valve to the fraction
collector's drop former. Follow the steps listed below, considering
your instrument's configuration.
• Solid Sample Cartridge with air purge enabled:
If you suspend the run with the Stop button, do not remove
the column and/or solid sample cartridge without first
pressing the Fast Forward button to purge solvents from all
lines. If you stop the run because your peak of interest has
been collected, protect the next user from being sprayed by
solvents left in the lines by pressing Fast Forward so the
instrument purges itself and is safe for its next use.
• Solid Sample Cartridge with air purge NOT enabled,
i.e., purge time set to zero in Method Editor:
If you suspend the run with the Stop button, remove the
column and install the Prime Tube in place of the column.
Manually purge the Sample Cartridge by pressing Tools ->
Manual Control -> Air Purge Cartridge. Repeat this
procedure for Air Purge Column.
• Liquid sample loaded onto column with air purge
enabled:
If you suspend the run with the Stop button, remove the
column and install the Prime Tube in its place. Use the Fast
Forward button to ensure no sample is left in the flow cell
and fraction collection lines, possibly affecting subsequent
separations.
CAUTION
Section 5 Operation
5-11
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