Table of Contents
Advertisement
+
CombiFlash® Rf
User Manual
3.11 Prime the Solvent Lines
Before the first use, the system should be primed. Ensure that
the solvent containers are filled, then:
1. Select the "Tools>Auto prime" menu command.
2. Click the Play button to start priming the system.
The system pumps 100% Solvent B through the system and
out the Waste port, and then repeats with 100 % Solvent A.
(This B-A order leaves the internal lines filled with solvent
appropriate for the beginning of a purification run.)
Note
When priming the first time, inspect the solvent and waste
connections to the system. If any leaks are observed, click the
Cancel button to stop the Auto Prime. Correct the leak by
tightening the fitting an additional
Prime from step 1.
To advance to the next Auto Prime step, you can click the
Fast Forward button. The Auto Prime window closes when
finished.
After Auto Priming, the system is ready for operation
(Section 5).
Use the Auto Prime feature for quick solvent changes by
pumping a fixed amount of solvent through the waste fluid
path at a high flow rate. For more advanced priming functions
such as varying the flow rates, fluid paths, etc., use the
Tools>Manual Control option.
3.12 System Verification
It is recommended that the system operation be verified. To do
so, use a pre-packaged CombiFlash Rf test kit:
• Part number 60-5237-050 — Test kit containing five of
the 5 gram size solid load cartridges pre-filled with 0.5
grams of test sample NPHE, five 4 gram RediSep Rf
silica gel columns, and instructions.
• Part number 60-5237-051 — Test kit containing four of
the 25 gram size solid load cartridges pre-filled with 0.5
grams of test sample NPHE, four 4 gram RediSep Rf
silica gel columns, and instructions.
3-18
1
turn, and then restart the Auto
4
Advertisement
Table of Contents
