Gradient Optimizer; Column Data - Teledyne CombiFlash Rf User Manual

button allows fractionation based on spectral purity.
Refer to the MONITORING THE PURITY MEASURE
topic for more details of these features.
Rf150 systems have a fixed UV detection wavelength of 254
nm. The peak detection settings for these systems include
slope-based and peak width options. These are described in 1
and 2 above. Rf150 systems with the variable wavelength
option include a control to type or set the detection
wavelength.

6.3.3 Gradient Optimizer

Use this window to automatically generate a gradient curve
from the results of two TLC plates. Follow the on-screen
instructions, then enter valid TLC data in the Gradient
Optimizer window. Click the OK button and the Method
Editor window will display the new gradient curve.
When using the Gradient Optimizer, the system may insert an
isocratic hold into the generated gradient. Furthermore, the
system may automatically extend the isocratic hold during the
run to increase peak resolution.
To open the Gradient Optimizer window, open the M
E
DITOR [6.3.2]
button.

6.3.4 Column Data

(Rf+ systems, including those equipped with 4x) The Column
Data window reports information about the RediSep Rf
column installed in the system. Compatible RediSep Rf and
RediSep Rf Gold columns have an RFID tag that identifies the
column type. The system reads this information to identify the
size and media, then automatically adjusts the default method
to match the inserted column.
If the column is reusable, the system will also write
information to the RFID tag that can help you manage its use.
The "Number of times used," "First used on," and "Last used
on" information can help you determine when the column
should be replaced. The "Last fluid used" will help you
determine if any solvent remaining in the column will be
miscible with the solvent currently used in the system.
To view the Column Data window, open the M
window, then click the Column Data button.
[6.3.2]
window, then click the Gradient Optimizer
Section 6 User Help Reference
ETHOD
ETHOD
E
DITOR
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