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CombiFlash® Rf
User Manual
4. To seal the column fittings, give the column a slight twist
1
(
turn).
4
Note
After loading a RediSep Rf column, the system (with the exception
of the Rf 150) will use RFID technology to automatically detect the
media type and column size. PeakTrak displays the detected
column size on the Main and Method Editor windows. If the
system does not detect the column, manually select the column
media and size.
5.3 Start a Default Method
After completing the system installation steps (Section 2
through 4, as necessary), preparing the sample, and inserting
the column, you are ready to perform a run with the default
method. The system's default methods are factory-set with run
parameters typically used by chemists. Default methods are
optimized for the use of RediSep Rf columns. The default
settings will:
• collect all fluid in the fraction collector rack
• differentiate between UV (254 nm) vs. non-UV absorbing
fluid and place the fluids into separate tubes
• cut UV absorbing peaks based on slope detection or level
threshold.
To start the run from the PeakTrak Main window:
1. Enter a sample name. If you choose not to, PeakTrak will
enter the date and time as the sample name when you start
the run.
2. Review the PeakTrak Main window settings. If you want to
change any settings, refer to the Editing a Method on-line
help topic.
3. Click the Play button.
4. Select the sample Loading Type from the list. This Loading
Type should support the sample preparation you chose in
Section 5.1. Possible sample loading types for the Rf
Lumen and Rf 4x systems are:
• Solid (Pause) – Select this option if you intend place
the sample into a solid sample load cartridge, but
5-6
+
, Rf
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