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CombiFlash® Rf
User Manual
1. After clicking the Cancel Run button, select the
Tools>Manual Control menu option.
2. From the Manual Control window (Figure 8-5), note the
Raw Lamp Energy level at 254 nm. The Raw Lamp Energy
gauge has three ranges: red, yellow, and green.
Figure 8-5 Raw Lamp Energy Gauge
• Red – lamp energy is obstructed to a degree that the
system might not reliably detect peaks. If you
attempt to operate the system, peak collection will be
forced to collect all. This prevents diverting desired
compounds to waste.
• Yellow – lamp energy is partially obstructed and the
peak detector performance might be impaired. The
system can be operated although the absorbance
trace might appear noisy. Yellow might be a normal
indication for systems with flow cell windows stained
by compounds.
• Green – lamp energy is sufficient to detect peaks up
to 2.4 Absorbance Units.
3. Remove the column and insert a bypass tube between the
upper and lower column mounts. A bypass tube (P/N
209-0165-46) can be found in the accessory kit shipped
with your system.
4. Set the Flow Rate to 40 mL/min (Figure 8-6).
8-8
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