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7.(Rf+ Lumen only) If the ELSD will be used for the run,
ensure that the ELSD option is selected. Otherwise, you
may disable the ELSD. The Rf+ Lumen system will
automatically check this option if the previous run used the
ELSD.
8.PurIon systems only. If the mass spectrometer will be used
for the run, ensure that the Mass Spectrometer option is
selected. Otherwise, you may disable the mass
spectrometer. The Rf+ PurIon system will automatically
check this option if the previous run used the mass
spectrometer.
9.PurIon systems only. Select "LOW" for highly loaded
columns or samples with a strong molecular ion to reduce
saturation of the mass spectrometer detector. Select
"HIGH" for lightly loaded columns of samples with weak
detection on the mass spectrometer. Default is "Medium".
10.Enter up to 4 ions, or a range and up to 3 ions. On PURION
S and PURION L systems only, the ions may be a mix of
positive and negative.
11.Click OK and the run begins. The system responds
according to the sample Loading Type you selected in
step 4.
Rf+ systems, including those equipped with 4x:
If you selected Solid (Pause), the system equilibrates
the column with the starting %B and then waits while
you prepare the solid sample load cartridge. Place the
cartridge on the system and click OK to continue.
If you selected Solid, the system equilibrates the
column and immediately starts the separation.
If you selected None (on column), the system skips the
equilibration and immediately starts the separation.
If you selected Liquid, the system equilibrates the
column and then waits. When instructed, perform the
following steps:
a. Place the injection syringe on the injection port.
b. Slowly force the liquid into the injection port.
c. Chase the first injection with a second injection
solution of at least 0.25 mL. The chase solution can be
the starting mobile phase or diluted sample mixture.
d. Click OK to continue the run.
Section 6 User Help Reference
6-45
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