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CombiFlash NextGen Systems
Section 3 Operation
3.1 Flash Sample
Preparation
3.1.1 Liquid Sample
Injection
3.1.2 Solid Samples
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
This section provides operating instructions for
CombiFlash NextGen systems.
Before starting a run, consider how the sample will be loaded
onto the column media. There are three different loading
methods: liquid injection, solid sample cartridges, and
pre-loading the sample on the column. Sample loading is covered
in the included CombiFlash NextGen Quick Start Guide.
If the sample is soluble in the starting mobile phase or a solvent
strength that doesn't significantly impact the separation, it can
be prepared as a solution and injected onto the column when
prompted during the purification run.
Some compounds are not soluble in solvents that are compatible
with the chromatography. In addition, the compounds may have
very limited solubility in any solvent, resulting in sample disso-
lution volumes that are impractical for good chromatography.
The answer in this case is solid sample introduction.
Some samples of this type may be simply dissolved and placed
into a pre-filled solid load cartridge and allowed to adsorb onto
the media in the cartridge. You can then use the cartridge imme-
diately or dry it before placing it on the system.
Other samples may need more care. Typically, you would dis-
solve the sample using a suitable solvent, then create a mixture
of ~20% sample load to media (w/w). Then dry the mixture under
conditions that will dry off the solvent without affecting the com-
pounds of interest. These compounds remain bound to the
media. Once dried, pour the media/sample mixture into an
empty cartridge.
3-1
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