Table of Contents
Advertisement
+
CombiFlash® Rf
User Manual
6.4.11.2 Air Purging
On CombiFlash Rf+ systems, including those equipped with
4x, you can purge the solid sample cartridge and column path
with air. To air purge a path, click the Air Purge Solid Sample
or Air Purge Column buttons. Click the Stop button to halt the
air purge.
6.4.11.3 Raw Lamp Energy
The right side of the Manual Control window shows a Raw
Lamp Energy gauge. This provides an indication of the UV
light measured by the optical detection system at 254 nm.
High lamp energy (green) means that the flow cell easily
passes through a sufficient UV light source. Lower lamp
energy (yellow or red) means that either the light source is
weak or that the flow cell is obstructed.
• Green – lamp energy is sufficient to detect peaks up to
2.4 Absorbance Units.
• Yellow – lamp energy is partially obstructed and the
peak detector performance might be impaired. The
system can be operated although the absorbance trace
might appear noisy. Yellow might be a normal indication
for systems with flow cell windows stained by
compounds.
• Red – lamp energy is obstructed to a degree that the
system might not reliably detect peaks. If you attempt to
operate the system, peak collection will be forced to
collect all. This prevents diverting desired compounds to
waste.
Depending on what you are doing with the Manual Control
functions, low lamp energy could be a normal indication. For
example, a UV-absorbing compound could be present in the
flow cell as you are pumping solvent. Or, the selected solvent
absorbs UV light at 254 nm. Abnormal indications could be a
flow cell blocked by a compound that has precipitated or there
is a film built up on the flow cell. If you suspect the low lamp
energy is an abnormal indication, perform the steps in the
Q
C
UICK
6.4.12 Setting the Vapor Limit
(Rf+ systems, including those equipped with 4x) The system
has an internal vapor sensor that detects vapors present in
6-62
LEANING WHEN
R
ECOMMENDED [
topic.
-
]
ON
LINE
Advertisement
Table of Contents
