Editing A Method - Teledyne CombiFlash Rf User Manual

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CombiFlash® Rf User Manual
Rf150 systems:
If you selected Equilibration, ensure that the solvent
delivery tubing is connected to the injection port above
the column. When you click the OK button, the system
equilibrates the column and then waits. When
instructed, perform a liquid injection as described in
steps a through d above, or attach the solid sample
cartridge to the injection port and connect the solvent
delivery tubing. Click the OK button to continue.
If you selected No Equilibration, ensure that the
solvent delivery tubing is connected. The system starts
the run immediately and assumes that the sample has
been injected on the column or the solid load cartridge is
in place.
You may allow system to proceed with the run while
monitoring the progress on the PeakTrak Main window. If
desired, gradient parameters may be modified during the run.
(See R
EAL
As the run progresses, the absorbance trace is drawn on the
Gradient Plot area. The purification continues until the end of
the run defined by the run length setting.
See Also:
R S
EDI EP
S
OLVENT
E
DITING A METHOD [6.4.2]
E
DITING A
B
YPASSING THE

6.4.2 Editing a method

Editing a method allows you to tailor the operation of the
CombiFlash Rf system to best separate or purify the
compounds of interest.
PeakTrak has two types of method settings — basic and
advanced.
6.4.2.1 Basic method settings
The basic settings allow you to control the %B gradient mix,
flow rate, solvents, and run length. These settings are part of
the M
AIN
frequently changed settings when developing or improving
purification methods.
6-46
- G
TIME RADIENT
R C S
F OLUMN ELECTION
S
ELECTION [
-
ON
D M
EFAULT ETHOD [6.4.4]
S L
OLID OAD
W . These settings are the most
INDOW [6.3.1]
E
DITING [6.4.6]
G
UIDE [
]
LINE
C
ARTRIDGE [6.4.7]
.)
- ]
ON LINE