Teledyne CombiFlash Torrent Quick Start Manual

®
CombiFlash
Quick Start Guide
Overview
These instructions supplement the
CombiFlash Torrent Module Installation Guide (part
number 60-5243-135) and briefly describe the steps nec-
essary to perform a purification run. These quick start
instructions assume that:
the system has been installed according to sec-
●
tion 2 of the Installation Guide,
the operator is familiar with the safety warnings
●
in section 1 of the Installation Guide, and
the system will be operated from the touch panel
●
display.
Quick Start Instructions
1. Perform Thin Layer Chromatography (TLC) on
the sample. From the TLC you'll learn of a suit-
able column media and solvent system, and the
difficulty of the compound separation.
2. With the column media known, select a
®
RediSep Rf column size appropriate for your
intended sample load. Refer to the Help topic
"RediSep Rf Column Selection Guide" for recom-
mended sample load ranges. If the separation is
difficult (R <0.2), your sample load should be
f
near the low end of the selected column's range.
3. Insert the RediSep Rf column on the
CombiFlash Torrent module. PeakTrak software
loads a method for that column. You can manu-
ally select the column media and size if the sys-
tem does not detect the column.
Note
Raising the upper column mount assembly from its
top- or bottom-most point of travel triggers the RFID
column detection. If you exchange a column without
fully lowering or raising the upper column mount,
select the File>New menu command instead to detect
the RediSep Rf column.
4. Ensure that the Solvent A and Solvent B lines are
routed to suitable solvents.
5. Select Tools>Auto Prime to open the Auto Prime
Window (Figure 1). Click the Play button. The
system primes the internal tubing and then
closes the Auto Prime window.
Torrent™
Instruction Sheet 69-5243-134
Figure 1: PeakTrak's Auto Prime window
6. On the main window, click the Play button.
7. From the Run Requirements window, select a
Loading Type.
If the sample is soluble in the starting mobile
❍
phase (Solvent A), select Liquid.
If the sample is not soluble in the starting
❍
mobile phase, select Solid (Pause).
If the sample is loaded directly on the column
❍
media, select None. The separation will begin
immediately without equilibration when you
click the OK button.
Note
Use the Solid option only when the solid sample
cartridge is already prepared and loaded on the
module. The module will equilibrate the column and
then automatically begin the run without user
intervention.
February 12, 2010