Table of Contents
Advertisement
8. Click OK and the run begins. The system responds
according to the sample Loading Type selected in step 4.
+
Rf
systems, including those equipped with 4x —
• If you selected Solid (Pause), the system
equilibrates the column with the starting %B and
then waits while you prepare the solid sample load
cartridge. Place the cartridge on the system and click
OK to continue.
• If you selected Solid, the system equilibrates the
column and immediately starts the separation. The
prepared solid load cartridge should be in place
before clicking the OK button.
• If you selected None (on Column), the system skips
the equilibration and immediately starts the
separation. The sample must be on the column before
clicking the OK button.
• If you selected Liquid, the system equilibrates the
column and then waits. When instructed, perform the
following steps:
a. Place the injection syringe on the injection port.
b. Slowly force the liquid into the injection port.
c. Chase the first injection with a second injection
solution of at least 0.25 mL. The chase solution can be
the starting mobile phase or diluted sample mixture.
d. Click OK to continue the run.
• PurIon Loading- (PurIon systems only) sets the
amount of sample sent to the mass spectrometer. Default
is "Medium". Select "LOW" for highly loaded columns or
samples with a strong molecular ion to reduce
saturation of the mass spectrometer detector. Select
"HIGH" for lightly loaded columns of samples with weak
detection on the mass spectrometer.
• DETECTION IONS- enter up to 4 ions, or a range and
up to 3 ions. On PurIon S and PurIon L systems, the ions
may be a mix of positive and negative.
• Mass Spectrometer- (PurIon systems only) click this
button to enable mass spectrometer detection
Section 5 Operation
5-9
Advertisement
Table of Contents
