During The Run; Run Control Buttons - Teledyne CombiFlash Rf User Manual

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CombiFlash® Rf User Manual
• ELSD- click this button to enable the evaporative light
scattering detector (Lumen systems only)
Rf 150 systems —
• If you selected Equilibration, ensure that the
solvent delivery tubing is connected to the injection
port above the column. When you click the OK
button, the system equilibrates the column and then
waits. When instructed, perform a liquid injection as
described in steps a through d above, or attach the
solid sample cartridge to the injection port and
connect the solvent delivery tubing. Click the OK
button to continue.
• If you selected No Equilibration, ensure that the
solvent delivery tubing is connected. The system
starts the run immediately and assumes that the
sample has been injected on the column or the solid
load cartridge is in place.

5.4 During the Run

You may allow system to proceed with the gradient while
monitoring the progress on the PeakTrak main window. If
desired, gradient parameters may be modified during the run.
(See the Real-time Gradient Editing on-line help topic.)
As the run progresses, the absorbance trace is drawn on the
Gradient Plot area. The separation or purification continues
until the end of the run defined by the run length setting.
During the run, data is automatically saved every few seconds.
PeakTrak automatically saves all run history on the system's
internal hard drive in its native .RUN format. You can
manually save a Run file as Text or PDF while stopped or any
later time as long as the .RUN file has not been deleted.
Selected files can be manually saved to a connected Flash
drive. To automatically save a Text or PDF file after each run,
configure the Network File Save feature (see Section 7.1.3).

5.4.1 Run Control Buttons

• Play — The Play button starts the run. During the run,
other run control buttons are active.
• Pause — The Pause button holds the %B at the current
value while the system continues to operate (sometimes
5-10