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CombiFlash® Rf
User Manual
4.Change the settings to meet your specific application
requirements. Refer to the E
instructions.
5.Click the Save button to replace the default method
settings.
You can reset the method to Teledyne Isco-recommended
defaults. While viewing the default method file for a selected
column size and type, click the "Reset file to Isco defaults"
button. The settings will revert to the original factory settings.
Click the Save button to save the settings for future use.
6.4.5 Defining a Gradient
The simplest way to change the gradient is to click and drag
the inflection points that define the shape of the gradient. Add
a point by clicking the Insert button, then click the gradient
curve to add a point. You can then drag the new point to the
desired location. Delete a point by clicking the Delete button
and then clicking the undesired inflection point.
The above methods work in the M
M
ETHOD
add, insert, and delete points using a tabular view on the
Method Editor window. Click the Edit Gradient button to open
this view, then use the controls to modify the settings. Click
the Edit Gradient button again to close the table view.
The system can also optimize a gradient from your TLC
results. To use this feature, select a single solvent system and
run two TLC plates, each with a different concentration of
solvent. For example, run one plate at 1:1 hexane:ethyl
acetate, the other at 1:3 hexane:ethyl acetate. If the resulting
Rf values are between 0.2 and 0.8, you can enter the TLC data
in the G
the data, the system will generate a gradient curve to separate
the target compound from the nearest impurity. When using
optimized gradients, PeakTrak may automatically insert an
isocratic hold to further increase the resolution.
6.4.6 Real-time Gradient Editing
The gradient shape can be changed during the run. Any time
after the fraction collector has positioned the drop former over
the first tube, click and drag the points on the gradient plot
area of the Main window. You can also add or remove points by
6-54
E
window. Alternatively, you can modify,
DITOR [6.3.2]
O
RADIENT
PTIMIZER [6.3.3]
DITING A METHOD [6.4.2]
W
AIN
INDOW [6.3.1]
window. When you accept
and
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