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3660 Quakerbridge Rd Trenton, NJ 08619 All rights reserved. No part of this publication may be reproduced by any means without the written permission of Teledyne Princeton Instruments. Printed in the United States of America. IntelliCal and SpectraSense are trademarks, and IsoPlane and LightField are registered trademarks of Teledyne Princeton Instruments.
® Chapter 1: IsoPlane 320A Description This manual is intended to help you set up and operate your new IsoPlane 320A spectrograph. Even if you are an experienced user of spectroscopic equipment, we suggest that you follow the manual (at least initially) to insure proper setup and operation. If you have any questions about the information contained in this manual, contact the Princeton Instruments customer service department.
1.1.1 External Power Supply Figure 1-2 illustrates a typical IsoPlane power supply with an inset providing the pin locations for the power connector. Refer to Table 1-1 for specific pinout information. Figure 1-2: IsoPlane Power Supply Table 1-1: IsoPlane Power Connector Pinout Information Pin Number Definition 1.1.2...
Chapter 1 1.2 Optional Accessories The following optional accessories are available for purchase for use with the IsoPlane system: Data Acquisition Software • The following data acquitision software is available for purchase from Princeton Instruments: ® LightField — ® The IsoPlane is supported by LightField 4.2, a 64-bit data acquisition platform for spectroscopy and imaging.
Filters; • Refer to Section C.6, Filter Wheels and Order Sorting Filter Assemblies, on page 67, for part number and ordering information. Fiber Optic Light Guides; • Refer to Section C.7, Fiber Optic Light Guides, on page 68, for part number and ordering information.
Chapter 1 1.3.1 External Connector Panel Figure 1-4 illustrates the indicators, interfaces, and associated connectors found on the External Connector panel. Figure 1-4: External Connectors POWER The following interfaces are accessible through this connector panel: FILTER • Filter driver connection required for an Acton FA-2448 Filter Wheel. SHT TTL •...
Chapter 2: Entrance Slit Accessories Princeton Instruments offers a full range of spectrograph accessories that mount directly to the IsoPlane’s manual or motorized entrance slit assembly. NOTE: Princeton Instruments spectroscopy accessories include customized installation procedures for their proper mounting and operation.
2.1 Mount an Accessory In general, the procedure to mount an accessory to the entrance slit is: Place the accessory directly against the face of the slit body. Using four (4) 8-32 screws typically provided with the accessory, secure the accessory to the slit body.
2.3 Slit Width Adjustments for Motorized Slits The Model 718-1 motorized slit assembly is a self-calibrating, 10 µm to 3 mm adjustable bilateral slit. Stepping-motor-controlled adjustments are made in 1 µm increments over the full range. The Model 718-12 motorized slit assembly is a self calibrating, 10 µm to 12 mm adjustable bilateral slit.
Chapter 3: Grating Turrets This chapter provides information about the installation and removal of grating turrets on an IsoPlane spectrograph. 3.1 Turret Installation If you have just received an IsoPlane spectrograph, you will need to install the grating turret. The grating turret has been shipped in the same shipping box with the IsoPlane but is packaged in a plastic container outside the IsoPlane housing to protect the gratings during shipment.
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Perform the following procedure to install a grating turret into an IsoPlane unit: CAUTION! Observe proper optical handling procedures. REFERENCES: Refer to Section 3.3, Turret Interchange, on page 21 for information about replacing one turret assembly with another. Locate the storage container with the grating turret that is to be installed. Using the 9/64”...
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Chapter 3 Grating Turrets Locate the hole near the edge between gratings on the grating turret assembly. See Figure 3-3. Figure 3-3: Location of Hole on Turret Grating Assembly Locate the vertical post on the Grating Spindle Assembly. See Figure 3-4.
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Carefully lower the turrent into the compartment. See Figure 3-5. Figure 3-5: Lowering Turret into Turret Compartment Position the grating turret on the grating spindle so the hole in the turret assembly aligns with the post in the turret assembly as shown in Figure 3-6.
Chapter 3 Grating Turrets Verify the turret is properly seated. Tighten the Center Torque Screw until it clicks. This sets the torque on the spindle correctly. NOTE: You cannot overtighten this screw. It is designed to slip once the correct torque is attained. Place the Turret Cover on the spectrograph.
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Carefully hand-loosen the center torque screw. See Figure 3-7. CAUTION! Use extreme care so as not to touch the grating surfaces. Figure 3-7: Grating Turret: Center Torque Screw ENTER ORQUE CREW Grasp the center torque screw and carefully lift the grating turret straight up off the grating spindle assembly.
Chapter 3 Grating Turrets Place the removed grating turret assembly into the original protective container in which it was shipped If a shutter assembly is not being installed or removed, or if a different turret is not being installed, replace the turret compartment cover. Replace the four (4) turret compartment cover screws and use a 9/64”...
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Figure 3-9: Grating Turret: Center Torque Screw ENTER ORQUE CREW CAUTION! Use extreme care so as not to touch the grating surfaces. Grasp the center torque screw and carefully lift the grating turret straight up off the grating spindle assembly. If the turret cannot be lifted up, the center torque screw may need to be loosened a bit more.
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Chapter 3 Grating Turrets Before removing the replacement grating turret from its shipping container, use a 3/32” hex wrench to loosen the three (3) set screws on the bottom of the container approximately one turn. Remove the cover from the shipping container, grasp the center torque screw, and turn it counterclockwise until the turret can be lifted straight up.
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Position the grating turret on the grating spindle so the hole in the turret assembly aligns with the post in the turret assembly as shown in Figure 3-13. Figure 3-13: Turret Installed with Hole and Post Aligned LIGNED OLE AND Verify that the turret is properly seated.
Chapter 4: Internal Shutter The IsoPlane has an optional internal shutter. If the shutter is installed, it is located in the Turret Compartment and is mounted to the spectrograph wall. Its shutter cable plugs into a keyed 2-pin shutter connector in the floor of the IsoPlane. The external connector is below the detector mounting plate and can be connected via a shutter cable to a detector with a SHUTTER connector.
4.1.1 Install an Internal Shutter Perform the following procedure to install an internal shutter: Using a 9/64” hex wrench, unscrew the four (4) socket head screws that secure the Turret Compartment cover to the top of the IsoPlane. Lift the cover and screws off of the IsoPlane and set them aside.
Chapter 4 Internal Shutter Figure 4-2: Connecting Internal Shutter HUTTER ABLE ONNECTOR HUTTER Carefully install the grating turret. Refer to Section 3.1, Turret Installation, on page 15 for complete information. CAUTION! Exercise extreme caution when handling the grating turret so as not to touch the grating surfaces.
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Reach into the compartment and disconnect the connector on the shutter cable from the connector mounted to the base of the IsoPlane by pulling it straight up. See Figure 4-3 Figure 4-3: Disconnecting Internal Shutter HUTTER ABLE ONNECTOR HUTTER Use a 9/64” hex wrench to loosen, but not remove, the two hex head socket screws that secure the shutter housing to the wall of the IsoPlane.
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Chapter 4 Internal Shutter Carefully slide the shutter up and off the two mounting screws to remove it. Place the shutter in a safe location for storage. Using the 9/64” hex wrench, tighten the two mounting screws so that they are snug and will not accidentally fall out.
Chapter 5: IsoPlane Setup This chapter provides information about the initial setup and configuration of an IsoPlane spectrograph. REFERENCES: For detailed information about the operation of an IsoPlane spectrograph, refer to Chapter 6, Operation, on page 43. 5.1 Unpack and Inspect Equipment Carefully unpack and examine the IsoPlane and all purchased accessories.
Install the grating turret that has been shipped in the same shipping box with the IsoPlane but is packaged outside the IsoPlane housing to protect the gratings during shipment. Refer to Section 3.1, Turret Installation, on page 15 for complete installation information.
Chapter 5 IsoPlane Setup Mount the detector to the plate and position the detector so the text on the back of the detector is readable (i.e., not upside-down.) NOTE: The exception to this text-orientation is the PI-MAX2: 1024x256 which should be mounted so that the text is upside-down. Use a 5/16”...
Launch the application software. Loosen the rotation adjustment screws (see Figure 5-2) sufficiently that you can rotate the detector. The mounting plate holes are slotted to allow approximately 4° of rotational positioning. If background subtraction is an available function, acquire a background to be applied while data are acquired.
Chapter 5 IsoPlane Setup Remove the Micrometer Compartment cover and set it aside. Using a 5/64” hex wrench, loosen the locking set screw shown in Figure 5-4. Figure 5-4: Micrometer Locking Set Screw OCKING CREW While continuously acquiring data from a spectral line source, adjust the micrometer until the half width of the peak is at a minimum.
If you have an optical fiber and fiber adapter that can precisely center the beam at the center of the IsoPlane focal plane, then that can be used as a guide when positioning the exit focal plane. If the IsoPlane is equipped with a standard 3 mm manual entrance slit or optional 3 mm motorized entrance slit, you can also install the supplied 4 mm high slit baffle and use the 4 mm high image as a guide when positioning the exit focal plane.
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Chapter 5 IsoPlane Setup Figure 5-5: Vertical Alignment Adjustment Set Screws NOTE: EPENDING ON THE DATE OF MANUFACTURE THE TWO ERTICAL LIGNMENT VERTICAL ALIGNMENT SCREWS DJUSTMENT CREW BE LOCATED IN THIS AREA ERTICAL LIGNMENT DJUSTMENT CREW CAUTION! When performing this procedure, be aware of, and adhere to, the following guidelines: •...
If the image is , alternately loosen the Cap Screw and tighten the Set Screw until HIGH the image is centered about the center row of the camera. CAUTION! Adjust ONLY the cap and set screw specified in Figure 5-5. Adjustments to other screws may result in severe misalignment of the instrument.
Chapter 5 IsoPlane Setup 5.6.2.2 Systems Equipped with a NON-Sliding Spec Flange Adapter A mirror adjustment should be performed ONLY after attempts to correct vertical alignment by adjusting the input image have failed. Horizontal adjustments are made by adjusting the pair of push-pull horizontal alignment screws as shown in Figure 5-6.
The following tools are required to perform this procedure: 5/32” hex wrench; • 3/32” hex wrench. • Perform the following procedure to adjust/fine-tune the horizontal alignment: If necessary, remove the Micrometer Compartment cover (see Figure 5-3) to access the height adjustment screws illustrated in Figure 5-6.
Chapter 5 IsoPlane Setup 5.8 Spectrometer Calibration Refer to the application software manual or the software help for information about calibrating the data pixels with wavelengths.
Chapter 6: Operation This chapter provides information about enabling the control of an IsoPlane and Princeton Instruments detector by either the LightField (64-bit) or WinSpec (32-bit) software. This chapter also points out how to perform alignment using the software. Additional information about operating the system can be found in the appropriate software manual or help file.
6.3 Using LightField Launch LightField after verifying that the detector and IsoPlane are both connected to the host computer and are turned on. When LightField boots it will look for available devices and will load their icons in the panel. Before you can design a new Available Devices experiment or run an existing one, the appropriate device icons must be dragged into the panel.
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Chapter 6 Operation Figure 6-2: Typical LightField Experiment Settings Stack and Experiment Devices Note that the stack on the left now displays several expanders. Experiment Settings Because this is a new experiment, the default settings for the detector will be active. The bar at the bottom of the window displays an icon for temperature status.
6.3.2 Alignment Since detectors are mounted directly to the mounting plate on an IsoPlane, the rotational alignment and focusing operations differ from the way these operations are performed for Acton SpectraPro Series spectrographs. NOTE: The following information assumes familiarity with the locations of the mounting plate, Micrometer Compartment, and the locking set screw.
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Chapter 6 Operation Figure 6-3: Typical Spectrometer Alignment Dialog Click on the peak that is to be monitored for the alignment. This will display the data cursor which can be positioned at the top of the peak. Since the data cursor spans the ROIs (graphs,) the data cursor can be used as a vertical reference.
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Figure 6-4: Micrometer Locking Set Screw OCKING CREW Click , and, while continuously acquiring data, adjust the micrometer until: The intensity level has been maximized, and • The FWHM for a selected peak or peaks has been minimized. • NOTE: function for the graph selected by the cursor is Peak Find automatically turned on to assist in identifying peaks and viewing...
Chapter 6 Operation 6.4 Using WinSpec After verifying that the detector and IsoPlane are connected to the computer and are both turned on, Launch WinSpec. WinSpec should automatically identify the detector that is to be used, and a dialog for selecting the detector for the experiment should be displayed. In addition, WinSpec must be instructed/configured to use IsoPlane.
6.4.2 Define the Spectrograph Perform the following procedure to configure WinSpec with the desired spectrograph: From the application’s menu bar, open the menu and select Spectrograph Define On the dialog, click on the Define Spectrograph Install/Remove Spectrograph button. Figure 6-7: Typical Define Spectrograph Dialog On the dialog, scroll through the list of supported Install/Remove Spectrograph...
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Chapter 6 Operation To verify that WinSpec recognizes the specified spectrograph, click Do Hardware . You should hear the turret moving. Initialize Figure 6-9: Typical Define Spectrograph Dialog: Acton 320A Active Once the spectrograph has been identified within WinSpec, the next step is to align the spectrograph optics to the detector.
6.4.3 Alignment The initial alignment procedure described in this section assumes: The detector and spectrograph have already been connected and turned on; • WinSpec has been launched and is running; • The detector and spectrograph have been identified/configured within WinSpec. •...
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Chapter 6 Operation Figure 6-11: Typical Move Spectrograph Dialog: Gratings Tab Set the slit to a minimum of 10 µm. NOTE: If necessary, adjust the Exposure Time to maintain optimum (i.e., near full-scale,) signal intensity. Wait until the detector locks at its default temperature. NOTE: When using an LN-cooled detector, fill the dewar after the detector has been powered on.
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Using the tab configure three 10-row ROIs as follows: Experiment Setup|ROIs One ROI at the top of the array; • One ROI in the middle of the array; • One ROI at the bottom of the array • Configure the data display on the dialog as follows: Display Layout is selected on the...
Appendix A: Grating Information Refer to Table A-1 for information about available gratings for an IsoPlane NOTE: Standard gratings measure 68 mm x 68 mm. Table A-1: Available Gratings Standard Part Mechanical Grooves/mm Blaze Wavelength Number Limit 600 nm I3-005-600-P 500 nm I3-015-500-P 11.2 µm...
A.1 Ordering Information Orders accepted subject to current pricing. All orders FOB Princeton Instruments-Acton, or shipping charges and insurance added separately. All orders subject to new customer approval. MasterCard/VISA MasterCard and VISA accepted for purchases. Electronic Orders Electronic orders accepted with proper Company Letterhead and complete contact information.
Appendix B: Camera Adapters This appendix provides detailed information about the following camera adapters available for use with an IsoPlane system: Sliding Spec Flange Mount [P/N: 9000-469-01]; • PyLoN Camera Mount [P/N: 9000-469]; • C-Mount Camera Mount [P/N: 9000-576]; • Special Spec Flange Mount [P/N: 9000-469-03].
Appendix B Camera Adapters B.2 PyLoN Camera Adapter The PyLoN camera adapter, part number 9000-469, is used exclusively with the PyLoN family of cameras. It allows rotational adjustments to be made to the camera’s positioning. This is an optional adapter available when ordering an IsoPlane system. Figure B-4: 9000-469 PyLoN Camera Adapter: Mechanical Drawing and Dimensions 7.000 6.375...
B.3 C-Mount Camera Adapter The C-Mount camera adapter, part number 9000-576, is compatible with all C-mount cameras in conjunction with MF-352. NOTE: The use of this adapter will introduce some vignetting when used with some cameras, such as the ProEM 1024. This is an optional adapter available when ordering an IsoPlane system.
Appendix B Camera Adapters B.4 Special Spec Flange Mount The Special Spec Flange mount, part number 9000-469-03, is used with the ProEM FT cameras. NOTE: This mount is currently not a standard part. Figure B-6: 9000-469-03 Special Spec Flange Mount: Mechanical Drawing and Dimensions 7.000 6.375 3.500...
Appendix C: Accessories This appendix provides detailed information about accessories available for use with an IsoPlane system. C.1 Slit Assemblies Refer to Table C-1 for information about available slit assemblies. Table C-1: Accessories: Slit Assembly Part Number Description SP-716-1 Bilaterally Adjustable Entrance Slit Assembly Micrometer adjustable widths from 10 µm to 3 mm.
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Table C-3: Accessories: Light Sources (Sheet 2 of 2) Part Number Description 110 V, 60 Hz 220 V, 50 Hz TS-425 TS-425-220 30 W Tungsten-Halogen Light Source Output wavelengths: 350 nm to >2.0 µm Includes housing, power supply, mounting flange, and light collection mirror.
Appendix C Accessories C.4 Detectors and Accessories Refer to Table C-4 for information about available detectors and detector accessories. NOTE: All detectors include mounting flanges for IsoPlane slit assemblies. Table C-4: Accessories: Detectors (Sheet 1 of 2) Part Number Description PD-438 Universal Photomultiplier Tube Housing For standard 1-1/8"...
Table C-4: Accessories: Detectors (Sheet 2 of 2) Part Number Description SI-440 General-Purpose Single Channel Detector 10 mm diameter active area Wavelengths: 400 nm - 1100 nm Enclosed in housing with the BNC connector. SI-440-UV Single Channel Detector UV-enhanced, 10 mm diameter active area Wavelengths: 200 nm - 1100 nm ID-441 InGaAs Detector...
Appendix C Accessories C.6 Filter Wheels and Order Sorting Filter Assemblies Refer to Table C-6 for information about available filter wheels and order-sorting filter assemblies. Table C-6: Accessories: Filters Part Number Description FA-2448 Six Position Filter Wheel Assembly Manual sample indexing, holds up to six samples, each of which is 1.0-inch in diameter. Automated via SP Control Cable.
C.7 Fiber Optic Light Guides All fiber optic light guides are comprised of 19 fibers, each of which is 200 µm in diameter. The fibers are arranged in a circle at the source end and a line at the slit end. See Figure C-1.
Appendix C Accessories C.8 Data Cables Refer to Table C-8 for information about available cables. Table C-8: Accessories: Data Cables Part Number Description CC-499-2 RS-232 Serial Cable 9-pin female to 9-pin male Length: 6 feet (1.82 m) 3650-USB-06 USB cable Type A to B Length: 6 feet (1.82 m NOTE:...
Appendix D: RS-232 Interface This appendix provides information necessary when using the RS-232 interface for communication between the host computer and an IsoPlane system. D.1 Communication Configuration Settings When using RS-232 for communication with the IsoPlane spectrograph, the RS-232 port on the host computer system must be configured as follows: 9600 Baud;...
Table D-1: IsoPlane RS-232 Pin Definitions DB9 Male DB9 Female Signal Definition Cable Color Pin Number Pin Number No Connection — Rx Data Tx Data No Connection — Ground Green No Connection — No Connection —...
Appendix E: Monochromator Control Software As has been previously discussed in this manual, the Acton IsoPlane spectrograph can be controlled using Princeton Instruments’ LightField, WinSpec, or SpectraSense software. It can also controlled from a computer using the supplied Monochromator Control software via the RS-232 or USB interface.
E.2 Connecting the IsoPlane to a Computer The IsoPlane has been designed to be operated and controlled by a host computer using the RS-232 or USB interface. IsoPlane operations which can be controled include: Wavelength scanning at a pre-set linear scan rate; •...
Teledyne Princeton Instruments will repair or replace, at its sole option, any defective parts, without charge to you. You must deliver the entire product to the Teledyne Princeton Instruments factory or, at our option, a factory-authorized service center. You are responsible for the shipping costs to return the product to Teledyne Princeton Instruments.
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Sealed Chamber Integrity Limited 12 Month Warranty Teledyne Princeton Instruments warrants the sealed chamber integrity of all our products for a period of twelve (12) months after shipment. If, at anytime within twelve (12) months from the date of delivery, the detector should experience a sealed chamber failure, all parts and labor needed to restore the chamber seal will be covered by us.
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Princeton Instruments. 2. You must notify the Teledyne Princeton Instruments factory service center within (30) days after you have taken delivery of a product or part that you believe to be defective.
Contact Information Roper Scientific's manufacturing facility for this product is located at the following address: Teledyne Princeton Instruments 3660 Quakerbridge Road Trenton, NJ 08619 (USA) Tel: +1 609.587.9797 pi.info@teledyne.com techsupport@princetoninstruments.com...
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Index Accessories LightField application software ....7 Cables ........69 Locking set screw .
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Tapered mounting plate......40 Technical support ......82 Turret installation.