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CombiFlash® R
Installation Guide
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Note
After loading a RediSep R
technology to automatically detect the media type and column
size. PeakTrak displays the detected column size on the Main and
Method Editor windows. If the system does not detect the column,
manually select the column media and size.
3.3 Start a Default Method
After completing the installation steps in Section 2, preparing
the sample, and inserting the column, you are ready to
perform a run with the default method. The system's default
methods are factory-set with run parameters typically used by
chemists. Default methods are optimized for the use of
RediSep R
• equilibrate the column
• collect all fluid in the fraction collector rack
• differentiate between UV vs. non-UV absorbing fluid
and place the fluids into separate tubes
• cut UV absorbing peaks based on slope detection or level
threshold.
To start the run from the PeakTrak Main window:
1. Enter a sample name. If you choose not to, PeakTrak will
enter the date and time as the sample name when you start
the run.
2. Review the PeakTrak Main window settings. If you want to
change any settings, refer to the Editing a Method on-line
help topic.
3. Click the Play button.
4. Select the sample Loading Type from the list. This Loading
Type should support the sample preparation you chose in
Section 3.1. Possible sample loading types are:
3-4
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columns. The default settings will:
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column, the system will use RFID
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