Protein Measurements; Making A Measurement - Thermo Scientific NanoDrop Lite Plus User Manual

Protein Measurements

Making a Measurement

Thermo Scientific
Purified protein samples can be quantified on the NanoDrop Lite Plus
spectrophotometer. To measure the concentration of a protein sample, select the
Protein application from the Home screen and then select the appropriate assay
type (1 Abs= 1 mg/mL, IgG, Lysozyme, BSA, or Other Protein).
1. Select Protein from the Home screen.
2. Select the appropriate assay type: 1Abs=1mg/mL, IgG, Lysozyme, BSA, or
Other Protein.
3. Following the on-screen instructions, establish a blank by pipetting 2 μL of the
blanking buffer onto the bottom pedestal, lower arm and select Blank.
4. When measurement is complete, raise the arm and wipe the buffer from both the
upper and lower pedestals using a dry laboratory wipe.
5. Confirm Blank by pipetting a fresh aliquot of blanking buffer onto the bottom
pedestal, lower the arm and select Measure. The resulting absorbance at the
analysis wavelength should be no more than 0.04 A. If your absorbance does
not meet this criteria, establish a new blank with a fresh aliquot of buffer.
6. When measurement is complete, raise the arm and wipe the buffer both the
upper and lower pedestals using a dry laboratory wipe.
7. Measure sample by pipetting 2 μL of sample onto the bottom pedestal, lower
arm and select Measure.
8. Wipe the upper and lower pedestals using a dry laboratory wipe and the
instrument is ready to measure the next sample.
Note Use a fresh aliquot of sample for each measurement.
When working with protein samples it is recommended that a 2 μL sample volume
be used to ensure that a liquid column is formed between the upper and lower
pedestals. This is especially true for concentrated samples or samples that contain
detergents. "Sample Volume Requirements"
1
NanoDrop Lite Plus User Guide
Protein Measurements
on page 17.
NanoDrop Lite Plus User Guide 13