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ACCQPrep HP125
Section 5 PeakTrak
5.3 Examples of PeakTrak
actions
5.3.1 Editing a method
5-26
4. 4.Click the OK button to save your settings and close the
window.
The following menu commands will require a password before
displaying the requested window:
• T
> U
M
OOLS
SER
ANAGEMENT
• H
> S
ELP
ERVICE
• T
> S
A
OOLS
ET
DMINISTRATOR
the current password.
In this section you will find instructions for typical PeakTrak
tasks, such as:
• EDITING A METHOD
• ALTERNATIVE WAYS TO CREATE METHOD FILES
• EDITING A DEFAULT METHOD
• DEFINING A GRADIENT
• REAL-TIME GRADIENT EDITING
• MONITORING THE PURITY MEASURE
• VIEWING RUNS
• MANUAL CONTROL OF THE ACCQPrep
Editing a method allows you to tailor the operation of the
ACCQPrep system to best separate or purify the compounds of
interest.
PeakTrak has two types of method settings — basic and
advanced.
Basic Method Settings – T h e b a s i c s e t t i n g s a l l o w y o u t o
control the %B gradient mix, flow rate, solvents, and run length.
These settings are part of the M
the most frequently changed settings when developing or
improving purification methods.
The %B gradient mix and run length are shown on the plot area.
Refer to Section 5.3.3 Defining a Gradient. for more details. The
other settings are:
• Flow Rate — this defaults to the value created when
setting up the HPLC column but can be changed for
each separation if desired.
• Solvent A and B — Select the desired solvents from the
lists. The solvents are those defined by the C
window.
RATION
Advanced Method Settings – The M
gives you access to advanced settings such as equilibration
volume, initial waste, air purge, peak detection, and peak col-
lection mode.
Equilibration Volume – Equilibration Volume Column equili-
bration is optional, but you may find that separations are more
effective if the column is equilibrated before injecting the sample.
P
. This protects
ASSWORD
window. These settings are
AIN
ONFIGU
E
ETHOD
DITOR
-
window
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Troubleshooting
