Default Run Units; Gradient Method - Teledyne Installation Guide Installation Manual

CombiFlash® R Installation Guide
f
To prevent unauthorized time changes, this feature is
password protected. The system is shipped with the password
set to combiflash. Use the Tools>Set Password menu
command to change this password for greater security.

2.11.8 Default Run Units

Run units are displayed along the X-axis of the chromatogram.
Select Time (in minutes) or Column Volumes. A column
volume unit is the time it takes to pump enough solvent to
exchange the volume held by the column. The duration in
minutes will vary according to the column media and size, and
the flow rate.

2.11.9 Gradient Method

Select the standard gradient type (Figure 2-8).
• Linear – The system gradually mixes solvents A and B
from the last programmed %B until it reaches the next
programmed %B. This method connects the inflection
points that define the gradient with a straight line.
• Step – The system holds each %B value until it reaches
the next programmed %B. Because the system holds the
%B values, the changes at each new value are nearly
instantaneous, producing a stepped appearance on the
plot area.
• Isocratic – The system holds the initial solvent mix for
the entire run.
Figure 2-8 Gradient types
2-16
Linear Step Isocratic