During The Run - Teledyne Installation Guide Installation Manual

CombiFlash® R Installation Guide
f
7. Click OK and the run begins. The system responds
according to the sample Loading Type you selected in
step 4.
If you selected Cartridge w/ pause, the system equili-
brates the column with the starting %B and then waits
while you prepare the solid sample load cartridge. Place the
cartridge on the system and click OK to continue.
If you selected Cartridge w/o pause, the system equili-
brates the column and immediately starts the separation.
If you selected Preload on column, the system skips the
equilibration and immediately starts the separation.
If you selected Liquid, the system equilibrates the column
and then waits. When instructed, perform the following
steps:
a. Place the injection syringe on the injection port.
b. Slowly force the liquid into the injection port.
c. Chase the first injection with a second injection solution
of at least 0.25 mL. The chase solution can be the
starting mobile phase or diluted sample mixture.
d. Click OK to continue the run.

3.4 During the Run

You may allow system to proceed with the run while
monitoring the progress on the PeakTrak main window. If
desired, gradient parameters may be modified during the run.
(See the Real-time Gradient Editing on-line help topic.)
As the run progresses, the absorbance trace is drawn on the
Gradient Plot area. The separation or purification continues
until the end of the run defined by the run length setting.
3-6