Preparing The Taqman Rnase P Verification Plate - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 7 Verifying the Instrument Performance
Preparing the TaqMan
Preparing the TaqMan
You must perform an ROI calibration
IMPORTANT!
(see page 35), a background calibration (see
and a pure dye calibration (see
running an RNase P plate.
Wear powder-free
IMPORTANT!
gloves when you handle the RNase P
Verification plate.
Retrieve the TaqMan RNase P Verification Plate
1.
Kit from the freezer, remove the RNase P plate,
then allow the plate to warm to room temperature
(approximately 5 minutes).
Remove the RNase P plate from its packaging.
2.
If the RNase P plate contains a
Note:
compression pad, remove it from the plate.
Applied Biosystems does not recommend the use
of compression pads with the 7300/7500/7500
Fast system.
Standard plates only: Vortex the plate for
3.
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
Centrifuge the plate for 2 minutes in a centrifuge
4.
with a plate adapter (<1500xg).
The plate must be well mixed and
IMPORTANT!
centrifuged.
Notes
90
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
®
RNase P Verification Plate
®
RNase P Verification Plate
page 49)
page 67) before
Ambient >00:05:00
<1500× g 00:00:05
RNase P
Verification Plate