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2.12 Prime the Solvent Lines
Before the first use, the system should be primed. Ensure that
the solvent containers are filled, then:
1. Select the "Tools>Auto prime" menu command.
2. Select the desired A and B solvents from the drop-down list
boxes.
3. Click the Play button to start priming the system.
The system pumps 100% Solvent B through the system and
out the Waste port, and then repeats with 100 % Solvent A.
(This B-A order leaves the internal lines filled with solvent
more appropriate for the beginning of a purification run.)
Note
When priming the first time, inspect the solvent and waste
connections to the system. If any leaks are observed, click the
Cancel button to stop the Auto Prime. Correct the leak by
tightening the fitting an additional
Prime from step 1.
To advance to the next Auto Prime step, you can click the
Fast Forward button. The Auto Prime window closes when
finished.
After Auto Priming, the system is ready for operation
(Section 3).
Note
Use Auto Prime for quick solvent changes by pumping a fixed
amount of solvent through the waste fluid path at 100 mL/min. For
more advanced priming functions such as varying the flow rates,
fluid paths, etc., or to purge the system with air before changing
between normal and reversed phase solvent systems, use the
Tools>Manual Control option.
Section 2 System Preparation
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1
turn, and then restart the Auto
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