Optical Filter Access Door; Photomultiplier Tubes - BIO RAD ZE5 User Manual

Chapter 2 Hardware Description

Optical Filter Access Door

Extra optical filters can be stored in the slots in the filter access door. The filter door includes a sensor
that communicates with Everest Software any time the door is opened for at least 5 sec.
The ZE5-EYE process runs in the background to check the detection paths any time the filter access
door is opened and then closed. It notifies system operators if any filter has been changed, so that the
filter configuration in the software can be updated accordingly.
For more information about the ZE5-EYE, see
135.

Photomultiplier Tubes

PMTs detect and amplify the scattered and fluorescent light signals produced by laser interrogation of
the particles. Located behind the optical filters, the PMTs detect specific bands of fluorescent light
based on the attached fluorochromes.
The ZE5 Cell Analyzer can be configured with a maximum of 30 PMTs that can be utilized
simultaneously, including:
n Forward scatter (FSC) detector
n
Optional second FSC detector
n
Side scatter (SSC) detector
n 27 fluorescence detectors
46 | ZE5 Cell Analyzer and Everest Software
Using the ZE5-EYE to Confirm Filter Choices on page