BIO RAD ZE5 User Manual page 357

Error
Noisy threshold plot
Possible causes
Debris in sample line
Debris in sheath or DI water
bottles
Dead cells in sample
Acquisition/Event Issues
Troubleshooting steps
Follow these steps, testing
after each to determine
whether the problem has
been resolved:
1. Run the cleaning process.
See Cleaning the Sample
Line and Probe on page
324.
2. Run the Decontamination
wizard. See
Decontaminating the
System on page 325.
Follow these steps, testing
after each to determine
whether the problem has
been resolved:
1. Run the cleaning process.
See Cleaning the Sample
Line and Probe on page
324.
2. Run the Decontamination
wizard. See
Decontaminating the
System on page 325.
Adjust gates (see Applying
Filters (Gates) on page 236)
or repeat experiment with
fresh cells.
User Guide | 355