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Chapter 9 Applying Fluorescence Compensation
3.
Click the position for the first control, in this case A2, and adjust the region in the histogram so that
it includes the stained population, as shown in the next figure.
4.
Repeat step 3 for all the control positions, adjusting the gate in the parameter's histogram if
needed.
5.
Click the down arrow next to the Compensation button.
6.
Select options as needed:
n
Prevent Automatic Region Determination — If this option is not selected, Everest Software
performs automatic region determination and adjusts the regions in all plots for each control
296 | ZE5 Cell Analyzer and Everest Software
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