BIO RAD Trans-Blot Turbo Instruction Manual
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BIO RAD Trans-Blot Turbo Instruction Manual

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Trans-Blot
Turbo
®
™
Blotting System
Instruction Manual
Catalog number
170-4155

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Summary of Contents for BIO RAD Trans-Blot Turbo

  • Page 1 Trans-Blot Turbo ® ™ Blotting System Instruction Manual Catalog number 170-4155...
  • Page 3 Bio-Rad Laboratories. Bio-Rad reserves the right to modify its products and services at any time. This user guide is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors, or for any damage resulting from the application or use of this information.
  • Page 4 Trans-Blot ® Turbo™ Blotting System...
  • Page 5 Doing so will void your warranties and can put you at risk for electric shock. Return the Trans-Blot Turbo instrument or cassettes to the factory (US customers) or to an authorized distributor (all other customers) if repairs are needed.
  • Page 6 Trans-Blot ® Turbo™ Blotting System...

  • Page 7: Safety Compliance

    * EN 61010 is an internationally accepted electrical safety standard for laboratory instruments. Bio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.
  • Page 8 Trans-Blot ® Turbo™ Blotting System...

  • Page 9: Specifications

    • No load detection • No cassette detection • End of run • Watt/hr limit Trans-Blot Turbo Cassettes Dimensions: (L x W x H) 20.2 x 16.0 x 4.5 cm Anode: Platinized titanium electrode plate Cathode: Stainless steel Weight: 1.5 lb Gel Compatibility Suitable for transfer of two mini format gels or one midi fomat gel per cassette.
  • Page 10 Note: Tray base is polyethylene terephthalate glycol (PETG) and can be recycled according to local regulations for class 1 plastics. Trans-Blot Turbo RTA Transfer Kits Membrane dimensions (L x W): Mini format (7.0 x 8.5 cm), midi format (13.5 x 8.5 cm) Membrane: 0.2 µm nitrocellulose, 0.2 µm PVDF, or 0.45 µm low fluorescent PVDF...

  • Page 11: Table Of Contents

    Table of Contents Bio-Rad Technical Support Safety Warning Labels Safety Compliance Electromagnetic Compatibility FCC Warnings and Notes Specifications Section 1: Introduction Unpacking and Setup Instructions Chemical Compatibility Safety/Cautions/Warning Warranty Section 2: Equipment and Reagents Overview 2.1 User Interface Section 3: Trans-Blot ® Turbo™ System Transfer Setup 3.1 Transfer Using Transfer Packs...

  • Page 13: Section 1: Introduction

    Bio-Rad’s Trans-Blot Turbo system accelerates the semi-dry blotting process without sacrificing performance. With the Trans-Blot Turbo system, transfer time is reduced to as little as 3 minutes, and the prepackaged transfer packs provide excellent transfer efficiency and reproducibility while eliminating the time and mess associated with traditional transfer methods.

  • Page 14: Unpacking And Setup Instructions

    • For your safety and for the protection of your Trans-Blot Turbo instrument, Bio-Rad suggests you routinely clean the instrument in accordance with the enclosed instructions and that you routinely check the contact pins located at the back of the instrument cavity, as outlined in Appendix A.

  • Page 15: Warranty

    EN 61010 certification, and create a potential safety hazard for the user. Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad or an authorized agent.

  • Page 16: Section 2: Equipment And Reagents Overview

    The reagents and consumables available in the transfer packs are also available in a ready-to-assemble format as part of the Trans-Blot Turbo RTA transfer kits. These kits provide the same optimized buffers and consumables as the transfer packs, but require wetting of the membranes and filters before use.

  • Page 17: User Interface

    • NEW mode. Create, run, and save a new protocol for your protein of interest • LIST mode. Select from either a list of Bio-Rad preprogrammed protocols optimized for a variety of protein and gel types or a list of user-defined protocols • TURBO mode.

  • Page 18: Section 3: Trans-Blot ® Turbo™ System Transfer Setup

    Protocols optimized for different protein and gel types are available in the Bio-Rad preprogrammed protocols in LIST mode. The system may also be used with Trans-Blot Turbo RTA transfer kits, which offer the same transfer speed and efficiency as the transfer packs, or superior performance to traditional semi-dry blotting consumables such as extra-thick filter paper and Towbin transfer buffer (speed and efficiency will be similar to standard semi-dry blotting techniques).
  • Page 19 Mini Transfer Pack (Single Mini Gel) Setup The packaging for the mini transfer pack has the two ion reservoir stacks in the left and right wells of the tray. The right well contains the stack for the anode (below the gel) and is layered with the transfer membrane (nitrocellulose or PVDF).

  • Page 20: Placing A Transfer Pack Into The Cassette

    The transfer packs are vacuum sealed. Check that the membrane does not lift away with the foil packaging lid. If necessary, hold the edge of the membrane and peel while opening the pack. Note: The base of the tray is PETG plastic and can be recycled according to local regulations for class 1 plastics.
  • Page 21 Midi Transfer Pack Mini Transfer Pack (for one midi format gel or (for one mini format gel) two mini format gels) a. Lay the ion reservoir stack with the membrane (anode stack) in the center of the cassette base. Ensure that the stack is not overlapping the green rubber molding in the base.

  • Page 22: Transfer Using Rta Transfer Kits

    Slide the cassette (with the dial facing up) into one of the Trans-Blot Turbo instrument bays until it makes contact with the magnetic interlock in the back of the instrument tub and you hear a click. The cassette can be inserted into the bays with or without power to the system.

  • Page 23: Transfer Using The Turbo Protocol

    Bio-Rad preprogrammed protocols under LIST > BIO-RAD > MIXED MW. 1. Turn on the Trans-Blot Turbo system using the switch located on the right side of the instrument. After an initial Boot screen, the system will proceed to the Home screen.

  • Page 24: Disassembling And Removing The Membrane

    5. Empty residual liquid from the cassette. If no additional transfer will be performed immediately, rinse the base and lid of the cassette with deionized water and dry them with a paper towel. 6. Turn off the Trans-Blot Turbo system with the power switch if it is no longer required. Trans-Blot ® Turbo™ Blotting System...

  • Page 25: Transfer Using Other Preprogrammed Protocols

    Bio-Rad preprogrammed protocols and user-defined protocol lists (Figure 16). Fig. 16. Protocol options. Pressing the Bio-Rad preprogrammed protocols button accesses the protocols described in Table 3. • STANDARD SD provides typical semi-dry transfer conditions for use with conventional semi-dry western blotting consumables (see Section 3.6)

  • Page 26: Transfer Using A New Protocol

    Fig. 18. Edit NEW protocol screen. To prevent damage to the cassette or instrument, the Trans-Blot Turbo system will terminate a transfer if 30 watts per hour is exceeded over the course of the run. Depending on the initial run conditions when designing a protocol, the system may display a message warning that the 30 watts/hour limit may be exceeded during the run.

  • Page 27: Transfer Using Traditional Semi-Dry Consumables

    Fig. 19. Proper assembly of blotting sandwich using traditional consumables. For futher information, refer to the Bio-Rad Protein Blotting Guide: A Guide to Protein Detection and Blotting, bulletin 2895, for detailed information. Bulletin 2895 can be downloaded from our website, www.bio-rad.com, as a PDF file, or contact Technical Support at 1-800-424-6723.

  • Page 28: Optimizing Transfer Conditions

    With Standard Semi Dry Components 25 V, 1.0 A, 30 min Refer to the Bio-Rad Protein Blotting Guide, bulletin 2895, for more information on optimizing electrophoretic transfer. The Protein Blotting Guide can be downloaded from our website, www.bio-rad.com, as a PDF file, or call Technical Support at 1-800-424-6723.

  • Page 29: Section 4: Appendices

    Section 4: Appendices Appendix A. Maintenance The Trans-Blot ® Turbo™ system requires little maintenance. After you finish using the Trans-Blot Turbo system, rinse each cassette, base, and lid in deionized water to remove residual salts and to prevent salt buildup. Air-dry or use a paper towel to dry the cassette.

  • Page 30: Appendix B. Troubleshooting

    Appendix B. Troubleshooting For more information on troubleshooting, see the Bio-Rad Protein Blotting Guide: A Guide to Protein Detection and Blotting, bulletin 2895. This can be downloaded from our website, www.bio-rad.com, as a PDF file, or call Technical Support at 1-800-424-6723.
  • Page 31 Swirls or missing bands; diffuse Poor contact between the membrane and the gel. Air bubbles (or excess transfers buffer when using traditional semi-dry consumables) remain between the membrane and gel. Use the blot roller to ensure that air bubbles or excess buffer are removed.

  • Page 32: Overview Of The Three Protocol Modes

    Overview of the Three Protocol Modes TURBO: 1 mini format or 2 mini format gels, or 1 midi format gel in 7 min; 1 Mini-PROTEAN ® TGX™ gel in 3 min Trans-Blot ® Turbo™ Blotting System...

  • Page 33: List

    LIST: Bio-Rad preprogrammed protocols or user-defined protocols Trans-Blot ® Turbo™ Blotting System...

  • Page 34: New

    NEW: User can edit protocol parameters and name, save, and run the protocol Trans-Blot ® Turbo™ Blotting System...

  • Page 35: Consumables And Related Products

    Trans-Blot Turbo RTA Tranfer Kit, PVDF, midi format (13.5 x 8.5cm), for 40 blotsl 170-4275 Trans-Blot Turbo RTA Tranfer Kit, LF PVDF, mini format (7.0 x 8.5 cm), for 40 blots 170-4276 Trans-Blot Turbo RTA Tranfer Kit, LF PVDF, midi format (13.5 x 8.5 cm), for 40 blots...
  • Page 40 Bio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 3065 7550 Life Science Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00...

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