Related Manuals for BIO RAD Bio-Plex Pro
Summary of Contents for BIO RAD Bio-Plex Pro
- Page 1 Instructions for Use for Bio-Plex Pro™ & Bio-Plex Pro II Wash Stations Revision B Attention! Use only the magnetic carrier which arrived with your instrument. See page 17 for more details.
- Page 2 Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 3 Tween is a registered trademark of ICI Americas, Inc., Wilmington, DE, USA • Decon and Neutracon are registered trademarks of Decon Laboratories Limited, Sussex, UK • HydroControl is a trademark of Tecan Group Ltd, Männedorf, Switzerland Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 4 Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 5: Table Of Contents
NSTALLING THE NSTRUMENT Bio-Plex Pro Installation Diagram......................14 Bio-Plex Pro II Installation Diagram......................15 Installing the Tubing Bracket (Bio-Plex Pro II only).................. 16 Selecting and Installing a Plate Carrier ..................... 17 Changing the Vacuum Configuration ......................18 .......................... 19 NSTALLING THE ICROPLATE .......................... - Page 6 Options Submenu............................62 Vacuum Filtration............................62 Bubble Sensor ............................63 ............................63 ROCEDURES Rinse ................................. 63 Prime ................................. 63 Empty Prime Tray............................63 MAINTENANCE AND CLEANING ........................65 ..........................65 LEANING ROCEDURES Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 7 Warnings, Cautions, and Notes The following types of notices are used in this publication to highlight important information or to warn the user of a potentially dangerous situation: Information Gives helpful information. Caution Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 8 Collect waste electrical and electronic equipment separately WARNING Risk of fire and explosion! Ethanol is flammable and when improperly handled can lead to explosions. Proper laboratory safety precautions must be observed. viii Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 9 Second Serial Number Träge (Slow Blow Fuse) torr Torr – Millimeter of Mercury (mmHg) TYPE Name and Type of instrument Universal Serial Bus Volt Volt Ampere WEEE Waste Electrical and Electronic Equipment Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 10 Basic Operation and Maintenance Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 11: Basic Operation And Maintenance
This section gives you instructions to configure, install, prime, and use the Bio-Plex Pro™ and Bio-Plex Pro II Wash Stations. You can perform your first plate wash simply by using these instructions and the onboard wash program. This section also contains references to more detailed information throughout this manual. -
Page 12: Microplate Requirements
*For a complete list of suitable plates, please contact Bio-Rad technical support. Only these recommended filter plates should be used with the Bio-Plex Pro II, when configured for standard Bio-Plex or other polystyrene bead based xMAP assays. Recommended microplates for vacuum filtration:... -
Page 13: Installing The Instrument
3. Connect the solution tubes to the corresponding liquid bottles. 4. Connect the waste tube to the waste bottle (do not kink the tubes). 5. IF your instrument is a Bio-Plex Pro II, install the tubing bracket as shown on page 16. -
Page 14: Bio-Plex Pro Installation Diagram
Basic Operation and Maintenance Bio-Plex Pro Installation Diagram Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 15: Bio-Plex Pro Ii Installation Diagram
Basic Operation and Maintenance Bio-Plex Pro II Installation Diagram Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 16: Installing The Tubing Bracket (Bio-Plex Pro Ii Only)
Basic Operation and Maintenance Installing the Tubing Bracket (Bio-Plex Pro II only) Your Bio-Plex Pro II is shipped with the liquid hoses attached. Attach the tubing bracket to the left side of the instrument, as shown in the pictures below. -
Page 17: Selecting And Installing A Plate Carrier
2. Push the plate carrier completely into the instrument, so that the front of the carrier is even with or past the Bio-Rad logo. 3. Lower the plate carrier. 4. Lower the manifold arm. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 18: Changing The Vacuum Configuration
LLD CHANNEL. EXIT to Primary menu (press EXIT OK EXIT until it is no longer an option) PROGRAM < > The instrument must be turned off and then back on for the change to take effect. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 19: Installing The Microplate
The power switch is located at the rear of the instrument, above the power cable connection. Make sure the instrument is plugged in using the provided power cable before you attempt to operate. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 20: Keypad Basics
A priming step must also be performed when switching to a different wash buffer. Caution Do not use your new instrument until you have primed both channels. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 21: Failure To Prime
Alternately you may carefully use a syringe to inject 1 - 2 ml of water directly into the L1 port of the instrument, reconnect the tubing, and run a prime procedure. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 22: Plate Washing Programs
Choose OK. SSSSSSSSSSSS Rows are represented by S (selected) or N (not selected). All rows should be selected. It is highly recommended that all wells are selected and the entire plate is washed. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 23: Which Wash Program Do I Use
V 200 x2 or V 200 x3 Third party kits which require 200 µl washes (Vacuum only) For all protocols, it is highly recommended that you wash the entire plate. See page 18 for details. Quick Guide Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 24 If you are not sure how much time has elapsed, run a PREVAC program to clear any residual buffer that may have wicked back into the wells before proceeding to the next step Vacuum protocols will not work if you do not have buffer in every well. Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 25: Rinsing
When the rinsing procedure is complete, the following message is displayed: RINSE DAY STOP END The manifold remains in the prime tray until END is pressed. Press STOP to abort the procedure (prime tray will not be aspirated). Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 26: Rinse Night
Leave the instrument on if you want to soak the manifold overnight (Recommended during periods of heavy use) • Shut the instrument off if you do not wish to soak the manifold (Rinse Night only) Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 27: Changing The Plate Carrier
The most important cleaning procedure for this instrument is rinsing the liquid system with distilled water at the end of each day. Changing the Plate Carrier See Page 16 for detailed instructions on selecting and replacing the plate carrier. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 28: Preventive Maintenance Plan
• Check the filter(s) in the liquid bottle(s) for particles and rinse liquid filter(s) with distilled water • Clean the plate carrier guide bar with 70% ethanol Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 29: Every Six Months
Note Clean the aspirating and dispensing needles periodically or immediately if they become clogged with particles or crystals. Yearly (Service Engineer Required) Yearly maintenance is performed by the service engineer. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 30: Reference Section
Provided you follow standard safety precautions this section is not required for normal operation. There is some information in this section for troubleshooting and advanced programming options. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 31: Instrument Details
(gloves, lab coat, and safety glasses) when emptying / handling a waste bottle. Inquire about appropriate collecting points and approved methods of disposal in your country, state, or region. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 32: Intended Use
Use antifoaming agents and concentrations as recommended by corresponding manufacturers. Intended Use The Bio-Plex Pro and Bio-Plex Pro II are instruments for strip-wise processing of microplates in the 96-well format. The instrument is designed for professional use only and should only be operated by trained personnel. -
Page 33: Configurations
The Bio-Plex Pro II is also supplied with a vacuum manifold plate carrier to perform wash steps for standard Bio-Plex assays and other non magnetic xMAP assays. In order to do this, you must install the vacuum manifold plate carrier and connect the appropriate tubing as indicated in Changing the Plate Carrier on page 23. -
Page 34: Instrument Specifications
Rad 96-well flat bottom plates (Cat. # 171025001). Residual volume Measured under following conditions: <= 4 µl / well, using Bio-Plex Pro flat-bottom plates and the recommended preloaded programs. (Optimized for high bead recovery and low assay %CVs.) Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 35 -20°C to 60°C (-4°F to 140°F) Relative Humidity Operation 20 % to 80 % non-condensing Storage 10 % to 80 % non-condensing Others Overvoltage category Pollution degree Method of disposal Electronic waste (infectious waste) Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 36: Description
About the Instrument Description The diagram below shows the main components of the instrument: Manifold Arm power Power ON Manifold Keyboard and Display Unit Guide Bar Prime Tray Plate Support Liquid System Diagram Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 37: Bio-Plex Pro Dimensions
About the Instrument Bio-Plex Pro Dimensions Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 38: Bio-Plex Pro Ii Dimensions
About the Instrument Bio-Plex Pro II Dimensions Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 39: Rear Panel Connections
The nameplate also shows the model name, the model (part) number, and the serial number. Use the provided power cord or a similarly-rated replacement cord only. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 40: Detailed Installation
Report any discrepancy immediately. 8. Check the instrument accessories against the packing list. Save packing materials for further transportation purposes. The instrument must be returned in the original packaging. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 41 9. CD with software and support material 10. 10 Bio-Plex Pro flat-bottom plates The Bio-Plex Pro II is also shipped in one carton, and contains everything from the above list, plus: 1. Located in the same small bag indicated in # 3 above. Not needed for Bio-Plex a.
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Page 42: Detailed Operating Instructions
Caution At the end of each working day, perform the Rinse Night procedure with distilled water to ensure the proper performance of the instrument and to prevent needles from becoming blocked. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 43: Complete Instrument Keypad Functions
(rinse, prime, and empty prime tray). The following features are available: • Preloaded programs for processing Bio-Plex and Bio-Plex Pro assays (also appropriate for other standard and magnetic xMAP assays) • 20 user definable washing programs, stored under program positions 1 to 20 •... -
Page 45: Alphabetical List Of Lcd Text With Explanations
Defining New Programs Alphabetical list of LCD text with explanations Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 46 Defining New Programs Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 47 Defining New Programs Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 48: Defining Custom Wash Programs
The entire wash program is performed on one strip of a microplate before proceeding to the next strip. The strip is processed for the defined soaking time, before proceeding to the next strip or group of strips. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 49: Washing Positions
Z-Position Overflow Z-Position Custom Z-Position Bottom Wash Position Diagrams MOVE OVER. MOVE CUST. Movement of Movement of manifold manifold Overflow Position Custom Position Bottom Position Bottom Position Movement Diagrams for Z-positions with MOVE Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 50: Aspirating Modes
The aspirating needles are set at two positions on the bottom of the wells (front edge and back edge). Aspirate Y Offset 1 Aspirate Y Offset 2 Front edge Back edge Crosswise Aspiration for Flat Bottom Wells Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 51: Drip Mode
The following example program should not be used as a standard wash procedure for cell washing as it is necessary to adjust the wash parameters, such as Z-position “MOVE CUST.”, dispensing and aspirating rates, head speed, etc., according to the cell type used. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 52 Select PROGRAM END? and press OK. PROGRAM END? EXIT OK When the final aspiration step appears, press NO to finish FINAL ASP defining the program. Example of a typical wash program for working with adherent cells Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 53: Defining New Programs
Clear a wash program from the instrument’s menu The Program menu has the following structure: PROGRAM < > CLEAR START DEFINE EDIT SHOW < > EXIT OK < > EXIT OK < > EXIT OK < > EXIT OK Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 54: Define/Edit A Program
Add a final aspiration step if necessary. FINAL ASP? The parameters for the final aspirate step can be selected. NO YES START Once the program has been defined, the Start menu appears again. < > EXIT OK Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 55: Process Step: Aspirate
Program End or a new cycle. Note Low residual volumes cannot be guaranteed if not all wells in a strip are filled with liquid. To prevent cross contamination, the head speed should be lowered. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 56: Process Step: Dispense
PY = Program Step Number incremented by 1 Press OK to define another dispense step or press – or + to select the next program step, Program End or a new cycle. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 57 Z-Position Overflow Z-Position Custom Z-Position Bottom Position Diagrams MOVE OVER. MOVE CUST. Movement of Movement of manifold manifold Overflow Position Custom Position Bottom Position Bottom Position Movement Diagrams for Z-positions with MOVE Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 58: Process Step: Wash
PY = Program Step Number incremented by 1 Press OK to define another wash step or press – or + to select the next program step, Program End or a new cycle. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 59: Process Step: Soak
PY = Program Step Number incremented by 1 Press OK to define another vacuum filtration step or press – or + to select the next program step, Program End or a new cycle. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 60: Show Program
If no final aspirate step was used, “CX CYCLEEND” appears. Press OK to view the cycle number. END OK Press END to exit the Show menu. Press - to return to the previous step. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 61: Clear Program
OFF. The Settings menu has the following structure: SETTINGS < > EDIT PLATES OPTIONS BUBBLE SENSOR < > EXIT OK < > EXIT OK < > EXIT OK Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 62: Edit Plates
VACUUMFILTRATION PROCESS CONTROL EXIT OK EXIT OK EXIT OK Vacuum Filtration See Page 18. Enabling/Disabling Vacuum. Note Make sure that the vacuum transport is mounted when the vacuum filtration option is enabled. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 63: Bubble Sensor
For details, see the Rinse section on page 24. Prime For details, see Priming the Instrument on page 20. Empty Prime Tray Navigate to empty EMPTY PRIME TRAY in the Procedures and Press OK. The prime tray will be aspirated. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 65: Maintenance And Cleaning
Basic Operation and Maintenance section. Caution If the manifold is not rinsed, the needles will become blocked. If this occurs, the manifold will need expensive repairs or will have to be replaced. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 66: Cleaning The Manifold
The bottles must be cleaned regularly depending on the applications, using a mild detergent. WARNING When handling waste bottles, it is advisable to adhere to applicable safety precautions (including wearing powder-free gloves, safety glasses, and protective clothing) to avoid potential infectious disease contamination. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 67: Liquid Or Foam Spills
The contents of waste bottle are potentially infectious, so it is important to wear protective clothing (gloves, lab coat, and safety glasses) when emptying OR handling a waste bottle. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 68: Replacing The Manifold
WARNING Prime the instrument without liquid to empty all liquid from the system, before removing the manifold. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 69: Removing The Manifold
2. Lift the manifold arm and remove the two screws that attach the manifold to the instrument using the Allen key provided. 2 screws 3. Carefully pull the tubing off the connectors on the rear of the manifold and remove the manifold. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 70: Installing The Manifold
6. Attach the black manifold guard plate with the 3 screws using the Allen key provided. 3 screws 7. Lower the manifold arm and prime the instrument before starting a wash program. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 71: Instrument Disinfection
Decon Neutracon (Decon Laboratories Limited) • Microcide SQ (Global Biotechnologies) • Ethanol WARNING Risk of fire and explosion! Ethanol is flammable and when improperly handled can lead to explosions. Proper laboratory safety precautions must be observed. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 72: Disinfection Procedure
6. After a minimum contact time of 10 minutes, repeat step 5 of this procedure. 7. Wipe the outer surfaces of the instrument dry. 8. Pack the instrument and its accessories. 9. Wash your hands with a mild detergent, and then disinfect them. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 73: Disposal Of Instrument
Disposal of the Instrument Please contact your local service representative before disposing of the instrument. Caution Always disinfect the instrument before disposal. Pollution degree 2 (IEC/EN 61010-1) Method of Disposal Contaminated Waste Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 74 • Make sure to treat this material according to the applicable safety standards and regulations. • Always clean and disinfect all parts before disposal. Dispose of packaging material according to local regulations. Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
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Page 75: Troubleshooting And Error Messages
INIT ERROR Press OK to remove the error message and the instrument returns to the standby mode. Check that the plate carrier is correctly inserted and that the plate carrier system is clear. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 76: Manifold Arm Error
RS485 Error This error is displayed if vacuum filtration is activated in the instrument settings on a Bio-Plex Pro instrument. (A different message will be displayed on a Bio-Plex Pro II.) RS485 TIME OUT Press OK to remove the error message and to return to the standby mode. -
Page 77: Pc (Process Control) System Error
No Aspiration at Vacuum Filtration Error The following error message appears on the display: NO ASP AT VACFIL Press OK to remove the error message and to return to the standby menu. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 78: Lld Errors
LLD setting. Remember to exit to the Primary Menu before performing this function. (Press EXIT until the word EXIT disappears.) If this does not solve the problem, please contact technical support. Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 79: Declaration Of Conformity
Declaration of Conformity Bio-Plex Pro and Bio-Plex Pro II Wash Stations... -
Page 80: Index
Options ..............62 Wash Programs Overview.............. 20 Quick Guide............23 Primary ..............20 Running ............... 22 Procedures ............63 Selecting.............. 23 Program ............... 53 Working Area Requirements ........40 Quick Guide ............24 Bio-Plex Pro and Bio-Plex Pro II Wash Stations... - Page 81 Bio-Plex Pro and Bio-Plex Pro II Wash Stations...
- Page 82 Bio-Plex Pro and Bio-Plex Pro II Wash Stations...