Waste Bottles; Fluidics Connections - BIO RAD ZE5 User Manual

Chapter 2 Hardware Description

Waste Bottles

The waste bottles receive the system fluid after it has run through the flow cell and waste lines. The
two waste bottles are distinguished by red caps and each hold 4 L; together, they provide about 8 hr of
continuous run time. Decontaminate the fluid collected in the waste bottles in accordance with
biohazard waste disposal guidelines.
Caution: Biohazard! Consult with your local safety officer or review local, state, and
federal regulations to ensure proper handling and disposal of biohazardous
substance.
You can add 400 ml of full-strength bleach to an empty waste bottle upon installation in the instrument
for a final concentration of 10% in the full waste bottle. Alternatively, you can add full-strength bleach
to a full waste bottle and allow it to sit to thoroughly decontaminate biohazardous material in the
waste.

Fluidics Connections

The ZE5 Cell Analyzer bulk fluidics chamber is located on the front of the instrument to the left of the
loader. The fluidics chamber holds four large and two small bulk fluidics bottles. Connections to these
bottles are depicted in the next figure.
30 | ZE5 Cell Analyzer and Everest Software