BIO RAD ZE5 User Manual page 197

Setting Up the Run List
High Throughput
When high-throughput mode is activated, the selected samples are aspirated continuously and each
sample is separated with a series of air and water boundaries depending on the selected inside wash
time.
For more information about high-throughput mode, see High-Throughput Mode on page 76.
Pause After
When Pause After is activated, acquisition stops after the assigned sample position and the system
waits for your input before continuing. This can be used to verify results achieved, or to set
compensation values before moving on to the next series of samples.
Tip: Activate Pause After at the last compensation control position so that compensation can be
calculated before returning to sample recording. The resulting compensation matrix is written to
the FCS files.
Positions in the plate map in which Pause After is activated appear as
Volumetric Counting
When Volumetric Counting is activated, the number of particles in a specified volume of each sample
can be verified. In addition, the following events occur for each selected sample:
n The value for the Volume sample stop option changes from 40 µl to 10 µl.
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