Optics; Lasers - BIO RAD ZE5 User Manual

Cell analyzer and everest software
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Optics

The ZE5 Cell Analyzer optics include lasers, mirrors, filters, lenses, and photomultiplier tubes (PMTs).
The mirrors, filters, and lenses shape and guide the laser light to the interrogation points in the flow
cell; they also focus and filter the laser light before it reaches the detectors. The PMTs detect scattered
and fluorescent light signal.

Lasers

The ZE5 Cell Analyzer can be configured with a selection of up to five lasers from the Coherent OBIS
line of lasers.
A typical configuration contains lasers of wavelengths and powers as follows:
n
355 nm (UV) at 50 mW
n
405 nm (violet) at 100 mW
Optics
User Guide | 35

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