BIO RAD ZE5 User Manual page 329

n Replace the additive and cleaner bottles with bottles filled with 200 ml diluted 1% bleach.
This section explains how to decontaminate the probe and prepare the bleach solution.
Tip: You can prepare the bottles while the system is decontaminating the probe.
To decontaminate the probe and sample lines
1. Prepare 100 ml of filtered bleach solution using a 0.2 µl filter flask or syringe filter.
2. Prepare 10 ml of 10% vol bleach solution in filtered water by adding 1 ml filtered bleach to 9ml
filtered DI water.
3. Fill a stat tube with 3.5 ml of the 10% filtered bleach solution and insert it into the stat tube
position.
4. On the Home window of Everest Software, click STAT Tube to start acquisition.
5. Run the stat tube acquisition with the filtered bleach solution for 5 min at 1 µl/sec.
6. After 5 min, stop the stat tube acquisition and remove the stat tube.
7. Insert a stat tube containing 4.0 ml of DI water into the stat tube position.
8. Run the stat tube acquisition with DI water for 5 min at 1 µl/sec to remove any bleach from the
outside of the probe.
To prepare the bleach bottles for decontamination
1. Obtain the following materials:
n
Two 4 L sheath bottles
n One additive bottle
n
One cleaner bottle
n 0.2 µm filtered bleach
n
Filtered DI water
2. Prepare each 4 L sheath bottle:
a. Add 25 ml of filtered bleach solution to each bottle.
b. Add 2.475 L of filtered DI water to each bottle.
c. Ensure that the liquid solution in each bottle is thoroughly mixed.
3. Prepare the additive and cleaner bottles:
a. Add 2 ml of filtered bleach to each bottle.
b. Add 198 ml of filtered DI water to each bottle.
Decontaminating the System
User Guide | 327