Pmt And Laser Controls - BIO RAD ZE5 User Manual

Chapter 7 Creating Experiments and Workspaces

PMT and Laser Controls

In this section of the Instrument Control panel, you can modify settings related to lasers and their
associated detectors.
Table 38. PMT and Laser Control Items
Item
258 | ZE5 Cell Analyzer and Everest Software
Function
Display filter numbers/detector names
between detector filter numbers and detector names for each of
the lasers in the system.
Load voltages — loads PMT voltages from a run list file that
you browse to and select.
Reset voltages — resets PMT voltages to default values.
Open/close laser shutter
When this button is gray, the laser is not delivering light to the
flow cell.
Note:
The shutter must be open to allow parameter
selection from that laser. The shutter automatically closes
if no parameters for the laser have been selected in the
Experiment Builder.
Edit laser power — allows you to specify power settings for
individual lasers, in steps, from 10 mW to the maximum power
output.
Neutral density checkboxes
is moved in front of the forward scatter detector. For more
information, see Optical Mirror and Filter Types on page
Select parameter for acquisition
and name acquisition parameters. If a parameter is not
selected, the box remains unselected, appears gray, and is not
acquired or included in the FCS data file.
— toggles
— controls the laser shutter.
— if selected, a 2.0 ND filter
— allows you to select
39.