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Appendix A Troubleshooting
Viewing Everest Software 2.0 FCS Files in FlowJo Software
FlowJo Software does not automatically recognize FCS files imported from Everest Software 2.0. To
view Everest Software 2.0 FCS files in FlowJo, you must configure the cytometer settings in FlowJo
Software to recognize the ZE5 instrument.
To configure FlowJo to recognize Everest Software 2.0 FCS files
1.
Open FlowJo Software and import an Everest 2.0 FCS file.
2.
On the FlowJo toolbar, click Instrumentation. You should see the ZE5 Cell Analyzer instrument
listed as ZE5.
3.
On the FlowJo toolbar, click Preferences.
4.
In the Preferences window, select Cytometers.
5.
In the left column, select GENERIC (ZE5).
6.
(Optional) In the Cytometer Identification section, change GENERIC to BIORAD_ZE5.
7.
In the Parameter Scale Settings section, clear the Custom log scaling and Custom linear scaling
checkboxes if they are selected.
8.
Enter the following parameters:
n
For Custom log scaling, set Min. value to 1, Max. value to 214748, and Divider to 10000.
n
For Custom linear scaling, set Min. value to 0, Max. value to 214748, and Divider to 10000.
9.
In the Transformation Settings section, select Enable Transforms and keep the default value (-10).
10. Click OK to save the changes.
342 | ZE5 Cell Analyzer and Everest Software
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