BIO RAD ZE5 User Manual page 358

Appendix A Troubleshooting
Error
Two or more populations are
present when expecting one
Unexpected fluorescence signal
356 | ZE5 Cell Analyzer and Everest Software
Possible causes
Gating inaccurate
Target protein expressed on
multiple cells
Inadequate cell preparation
Large number of doublets in
sample
Free dye accumulating in sample
line
Troubleshooting steps
Adjust gates. See Applying
Filters (Gates) on page 236.
Evaluate sample preparation
and experiment setup.
Ensure adequate cell
separation and preparation
because multiple cell types
or debris could be present in
a sample.
Adjust the flow rate down.
Run the cleaning process.
See Cleaning the Sample
Line and Probe on page 324.