BIO RAD ZE5 User Manual page 394

Glossary
flow cell
heat map
height
high-throughput mode
hydrodynamic focusing
interrogation point
longpass filter
neutral density filter
PMT
pulse
pulse width
run list
392 | ZE5 Cell Analyzer and Everest Software
The fused silica chamber through which sample flows to be interrogated. Also
referred to as a cuvette.
A graphical representation of data where the individual values are represented
as colors or color shades.
The height of the pulse, as analyzed by the electronics.
A sampling mode in which the probe moves from sample to sample and
continuously aspirates sample, with programmed washes in between, resulting
in multiple samples in the sample line at the same time. In this mode, samples
cannot be returned to positions and reagent cannot be added to samples.
In the ZE5™ Cell Analyzer, the sample is injected into the middle of the sheath
fluid flow at the base of the cuvette just before the interrogation point. The two
fluids form a two-layer stable flow, centering the sample in the middle of the
sheath (carrier) fluid without mixing of the fluids.
The spot at which the lasers intersect the sample core inside the flow cell.
An optical component, usually placed in front of a detector, that allows
passage of a range of wavelengths of light above a specified wavelength while
absorbing or reflecting the rest.
An optical filter that reduces or modifies the intensity of all wavelengths of light
equally by reflecting or absorbing a portion of it.
Photomultiplier tube. An extremely sensitive vacuum phototube that detects
and amplifies the scattered and fluorescent light signals produced by laser
interrogation of particles.
The signal coming from a single particle in a single channel as it passes
through the interrogation point. The pulse is amplified by the PMT and
processed by the electronics.
The width of the pulse, as analyzed by the electronics.
A set of instructions — including media type, sample well/tube positions,
sample volume, limits, speed, wash and agitation, sample names, enabled
parameters, plots, and voltages — that allows the ZE5 Cell Analyzer system to
acquire a set of samples. Also referred to as an experiment.