Replacing Sheath Additive And Cleaner Bottles - BIO RAD ZE5 User Manual

Chapter 6 Daily Routine
Notice the 90 μm filter at the bottom of the fluidics line.
4. Refill the bottles with DI water or replace them with new bottles.
5. Carefully place the cap assemblies onto the bottles and tighten the caps.
Important:
n
Ensure that the filter falls to the corner of the bottle diagonally opposite from the cap, so that
the entire contents of the bottle can be drawn into the instrument without risking introduction
of air.
n
Ensure that the bottle cap is firmly tightened; otherwise, leakage might occur.
6. Place the sheath bottles back into the instrument.
7. Attach the cap assembly tubing to the connectors on the instrument.
Tip: An audible click indicates that the tubing is connected.

Replacing Sheath Additive and Cleaner Bottles

The sheath additive and cleaner bottles are located at the bottom of the fluidics chamber. They can be
refilled or replaced at any time other than during shutdown.
Sheath additive and cleaner bottle connections are specifically keyed to their respective ports and
cannot be connected to the wrong ports: the white-capped sheath additive bottle always goes on the
left and the blue-capped cleaner bottle always goes on the right.
Important: When filling the sheath additive or cleaner bottles:
n Fill the bottle only to the fill line to ensure that liquid does not leak from the vent in the cap.
n
Ensure that the tubing that extends into the bottle is not contaminated during the refill procedure.
To refill an additive or cleaner bottle
1. Push the quick connect tab on the connection to disengage the tube from the instrument.
148 | ZE5 Cell Analyzer and Everest Software