Acquisition/Event Issues - BIO RAD ZE5 User Manual

Appendix A Troubleshooting

Acquisition/Event Issues

Error
Acquisition pauses with track
region set
Air bubble warning
Data suddenly disappear from the
plots in the workspace and
threshold plot, and the event rate
drops to 0 during acquisition
344 | ZE5 Cell Analyzer and Everest Software
Possible causes
Clog causing events to fall below
specified track region percentage
Probe too high and not in sample
fluid
Trigger channel voltage too low
Troubleshooting steps
Follow these steps, testing
after each to determine
whether the problem has
been resolved:
1. Run the unclog process.
See Unclogging the
Sample Line and Probe on
page 322.
2. Run the cleaning process.
See Cleaning the Sample
Line and Probe on page
324.
Follow these steps, testing
after each to determine
whether the problem has
been resolved:
1. Check the level of QC
calibration beads and
replace the bead bottle if
needed. See Replacing the
QC Beads on page 335.
2. Recalibrate the probe,
using custom media
settings if needed. See
Media Selector on page
69.
Increase the trigger channel
voltage until data start to
appear in the threshold plot.
Then, adjust the trigger
voltage and threshold value
until data appear as expected
in the plots in the workspace.
See Configuring Instrument
Settings on page 257.