Chapter 8 Acquiring Samples
3.
Press the silver sample chamber button to retract the loader.
Setup Mode Controls
Use Setup mode to determine optimal instrument settings for sample acquisition. You can modify
acquisition parameters (including target flow rate or event rate, stop conditions, agitation, and wash
settings) from what was defined in the Experiment Builder. In Setup mode, you can adjust the
threshold and PMT voltages and create gates. You can also use the Record function to manually save
data to files. This gives you more control over when data are saved during the run. Data are only
saved after you click Record. Recording stops after the stop conditions have been met or when you
manually stop recording.
For a stat tube, the Setup button does not appear; sampling occurs in Setup mode by default.
Tip: To edit an experiment, navigate to the Instrument Control panel in Setup mode and click Edit.
276 | ZE5 Cell Analyzer and Everest Software
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