BIO RAD ZE5 User Manual page 42

Chapter 2 Hardware Description
n Longpass filters allow light above a determined wavelength to pass through while reflecting the
rest. For example, a 600 dichroic longpass (DLP) filter allows light with a wavelength longer than
600 nm to pass through, reflecting the rest.
n
Shortpass filters allow light below a determined wavelength to pass through while reflecting the
rest. For example, a 470 dichroic shortpass (DSP) filter allows light with a wavelength shorter than
470 nm to pass through, reflecting the rest.
n
Bandpass filters allow light within a narrow wavelength range width to pass through while
rejecting (attenuating) light of other wavelengths. For example, a 447/60 bandpass (BP) filter
allows light from 417 nm to 477 nm through, absorbing the rest.
n Neutral density filters reduce or modify the intensity of all wavelengths of light equally by reflecting
or absorbing a portion of it.
For experimental applications where events appear off scale when the detector is set at minimum
gain, a neutral density filter can attenuate the signal and keep the events on scale. In the ZE5 Cell
Analyzer, neutral density filters are used in front of the FSC detector. You also have the option to
install neutral density filters in front of other detectors.
For information about installing neutral density filters in front of detectors other than the FSC detector,
see Installing a Neutral Density Filter on page
see Replacing Optical Filters on page
When replacing optical filters, note the following dimensions and tolerances.
Table 4. Optical filter sizes
Filter type
Dichroic
Bandpass
* Tolerance on length and width : +0.0 mm/–0.2 mm.
** Tolerance on thickness : ±0.1 mm.
40 | ZE5 Cell Analyzer and Everest Software
139. For information about installing other filter types,
130.
Size (L* x W x thickness**)
17 x 15 x 1.05 mm
13 x 13 x 2 mm