Assigning Data Track Regions - BIO RAD ZE5 User Manual

Chapter 7 Creating Experiments and Workspaces
To remove a gate limit
1. Point to a plot or histogram that contains the gated region(s).
The plot and histogram toolbar appears.
2. Click Assign Gate Limit Regions.
3. In the Available Gate Limit Regions area that appears, clear the checkbox for each region from
which to remove a gate.
4. Point outside the plot or histogram to save your changes.
The gate symbol is removed from the region(s) and the gate limit no longer applies.
Tip: You can also assign gate limits using the Batch section of the toolbar in the Settings window.
This applies the specified limit to the specified gate in Setup positions (compensation controls),
Sample positions, or selected positions.
For more information on using this tool, see

Assigning Data Track Regions

You can use data track regions to monitor for clogs or sample disturbances during acquisition. When
you assign data tracking to a region in a density plot, you specify a target percentage. During
acquisition, if the percentage of events drops below the target, acquisition pauses and you are notified
so that you can determine what might be causing unexpected results. Data tracking is especially
useful in high-throughput sampling mode.
Note: When assigning data tracking to quadrant regions, wait until the experiment has been
applied to the workspace.
To assign data tracking to a region
1. Point to a density plot that contains the region where you want to use data tracking.
The plot and histogram toolbar appears.
2. Click Assign Data Track Regions.
3. In the Available Track Region area that appears, select the region where in which to track data.
242 | ZE5 Cell Analyzer and Everest Software
Creating Plots for the Experimental Sample on page 366.