Related Manuals for BIO RAD QX200
Summary of Contents for BIO RAD QX200
- Page 1 QX200 Droplet Reader, IVD and ™ QuantaSoft Software ™ Instruction Manual 186-3007 12001045 17000034 Manual revision: B Software revision: 1.7...
- Page 3 Bio-Rad Technical Support For help and technical advice, please contact the Bio-Rad Technical Support department. In the United States, the Technical Support department is open Monday–Friday, 5:00 AM–5:00 PM, Pacific time. Phone: 1-800-424-6723 Fax: 1-510-741-5802 Email: LSG_TechServ_US@bio-rad.com (for U.S. and international customers) Online technical support and worldwide contact information are available at www.consult.bio-rad.com.
- Page 4 Do not position the equipment so that it is difficult to operate the plug of the power supply. The plug of the power supply is the disconnect device. No serviceable parts inside. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual...
- Page 5 This product weighs in excess of 50 lb. When lifting, two people are required. Grip from the underside with the people on opposite sides. After unpacking for placement of equipment, transport per lifting instructions above; place near grounded outlet with plug of power supply accessible. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual...
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Page 7: Table Of Contents
1.3 Droplet Digital PCR Workflow ........4 1.4 System Setup and General Operation Instructions ......4 Chapter 2. Using the QX200 ™... -
Page 9: Chapter 1. Qx200 ™ Droplet Digital ™ Pcr System, Ivd
Viral load determination GMO detection ■ ■ ■ ■ For instructions on use of the QX200 Droplet Generator, IVD, please refer to the QX200 Droplet Generator, IVD Instruction Manual (bulletin 10000046997). QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 10: System Components
Intended Use and Indication for Use The QX200 Digital Droplet PCR System, IVD is a digital polymerase chain reaction system intended to measure and sort multiple signals generated in an in vitro diagnostic assay from a clinical sample. This instrumentation is used with a specific assay to measure multiple similar analytes that establish a single indicator to aid in diagnosis. - Page 11 Chapter 1 QX200 Droplet Digital PCR System, IVD QX200 Droplet Digital PCR System, IVD. Left, QX200 Droplet Reader, IVD. Right, QX200 Droplet Generator, IVD. Table 1.2. Additional materials required. Component Recommended Catalog # and Supplier QX200 Droplet Generator, IVD Reagents for probe detection...
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Page 12: Droplet Digital Pcr Workflow
■ ■ PX1 Plate Sealer (see Table 1.2). Perform PCR to end point (~40 cycles) using a thermal cycler Read droplets — load the plate into the QX200 Droplet Reader, IVD and start your run. The droplet reader sips ■ ■... -
Page 13: Chapter 2. Using The Qx200 Droplet Reader, Ivd
System, IVD. If any component not marked IVD CE is used, including reagents or accessories, the use must be research only. 1. Power on the QX200 Droplet Reader, IVD using the switch at the back. Allow it to warm up for 30 min, then switch on the PC and launch QuantaSoft ™... - Page 14 Confirm the first three indicator lights are green (Table 2.1). Push to open/close Placing the plate holder into the droplet reader. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual...
- Page 15 6. When droplet reading is complete, all four indicator lights are solid green. Open the door and remove the plate holder from the unit. Remove the 96-well PCR plate from the holder and discard it. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual...
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Page 17: Chapter 3. Using Quantasoft Software
Results (*.qlp) — user-defined plate layout settings and processed data for a ■ ■ ddPCR plate Comma-separated values (*.csv) — analyzed data in a format that can be ■ ■ assessed using other programs, such as Microsoft Excel QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 18: Setup
2. Enter the file name, then use the well editor (see Section 3.1.1) and experiment editor (see Section 3.1.2) to adjust the settings for your experiment. Click Options to access advanced options data analysis and setup (see Section 3.1.3). QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 19: Using The Well Editor
To create or edit an experiment, use the experiment editor (see Section 3.1.2) ■ ■ 3. Select the Supermix from the drop-down menu (required; selection cannot be changed after data collection). QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 20: Using The Experiment Editor
(see Section 3.3). Copy number options appear for CNV experiments only Customize the color codes for experiments (for example, by experiment type, user, etc.) Experiment editor. Options for a CNV experiment are shown. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 21: Using The Advanced Options
Active and analyzed wells are also highlighted in green in the plate map. 4. As each well is analyzed, the data appear across the top navigation area. Once the run is complete, all data are reanalyzed for the final data file. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 22: Analyze
Well selector — enables selection of wells for targeted analysis ■ ■ Processed data/graphical display — allows visualization of graphical data from selected wells ■ ■ QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... - Page 23 Export data to .csv file Toggle order in which channel data are displayed in the table Select data from detector channel(s) Results table options. Data from a CNV analysis are shown. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual...
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Page 24: Viewing Channel Data (1D Amplitude)
Set Threshold or Enter Click Auto Analyze to revert to automatic threshold settings and calculations. Threshold adjustments can also be made in the 2D Amplitude clustering plots (see Section 3.3.2). QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... - Page 25 (the threshold is indicated by the pink line and the value in the Set Threshold field; the status of that well in the results also shows Manual). In Channel 2, the multiple threshold tool is enabled. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual...
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Page 26: Viewing Clustering Plots (2D Amplitude)
Viewing clustering plots. Threshold adjustment options available in the clustering plot are shown. Threshold values are indicated by the pink lines in the plot. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 27: Viewing Concentration Data (Concentration)
(in copies per µl) multiplied by the 20 µl ddPCR reaction input used to make droplets. This value does not appear for merged well data. This column is also exported in the *.csv file. The original concentration column and calculations remain unchanged. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 28: Viewing Copy Number Data (Copy Number)
Viewing ratio data. Data from absolute quantification are shown. Hover over data points to reveal well identity, concentration, and Poisson confidence limits. Select y log to convert the y-axis to logarithmic scale (shown). Solid data points (not shown) indicate merged data; open data points (shown) indicate data from single wells. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 29: Viewing Events
The simulation mode also allows you to load a demo plate or create a new template and simulate a ddPCR run. ■ Options available in simulation mode Simulation mode (main screen). QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 31: Chapter 4. Specifications And Maintenance
Installation category II (external power supply plugs into standard AC receptacle) Ventilation requirements 5" (12 cm) left and right of machine and 10" (25 cm) behind should be unobstructed for proper ventilation QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 32: Maintenance
4.2 Maintenance 4.2.1 General Maintenance Procedures The QX200 Droplet Reader, IVD is verified by Bio-Rad to be functioning to manufacturer specifications upon installation. Yearly preventative maintenance offered by Bio-Rad is recommended to ensure the QX200 Droplet Reader, IVD continues to function according to manufacturing specifications. -
Page 33: Replacing Droplet Reader Oil And Removing Waste
Replacing droplet reader oil and removing waste. Slide out the carriage containing the droplet reader oil and waste receptacle to remove and replace the bottles (left). After replacing oil, click Prime (right) to fill the lines; other instrument maintenance routines are for use by field specialists only. QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 34: Maintenance Routines
■ ■ Move to Waste — to move the sample needle to the waste receptacle ■ ■ Custom — to run a custom routine (*.qtl); this option is password-protected ■ ■ QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... -
Page 35: Appendix A Ordering Information
Droplet Generator, 1 pkg of 24 gaskets (5 x 1 ml), 2x supermix 186-3051 DG8 Cartridge Holder 186-3027 ddPCR Supermix for Probes, 25 ml (5 x 5 ml), 2x supermix 29711024 Droplet Reader Plate Holder QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual... - Page 36 50 ml (10 x 5 ml), 5,000 x 20 µl reactions 186-3052 ddPCR Buffer Control Kit for Probes, 2 x 4.5 ml bottles, 2x buffer 186-4052 ddPCR Buffer Control Kit for EvaGreen, 2 x 4.5 ml bottles, 2x buffer QX200 Droplet Reader, IVD and QuantaSoft Software Instruction Manual...
- Page 40 Bio-Rad Laboratories, Inc. 5731 W. Las Positas Blvd Pleasanton, CA 94588 Bio-Rad 3, boulevard Raymond Poincaré 92430 Marnes-la-Coquette, France Tel.: + 33 (0)1 47 95 60 00 Fax: + 33 (0)1 47 41 91 33 www.bio-rad.com Bio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 43 1 877 89 01 Life Science Belgium 03 710 53 00 Brazil 55 11 3065 7550 Canada 905 364 3435 China 86 21 6169 8500 Group...
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