Assigning Position Types - BIO RAD ZE5 User Manual

Setting Up the Run List
Reagent Settings
Before you can specify addition of reagent to any samples, first designate one or more positions in the
plate map as reagent positions. Then, for a selected sample position, you can select a reagent
position from the dropdown list and enter the required volume of reagent to add to the sample, in the
range of 2–20 µl. You can instruct the system to mix the reagent after it is added by aspirating and
dispensing the combined sample and reagent several times. You can also choose to add an agitation
step to the position to enhance mixing.
Positions in the plate map on which reagent will be added appear as

Assigning Position Types

Tip: This procedure explains how to assign position types for a single-panel experiment. If
needed, you can set up a single plate or tube rack with multiple experimental panels. For more
information, see Multipanel Experiments on page 87 and Setting Up Multiple Panels on page
262.
Assign positions based on their type: Wash, Sample, Reagent, or Setup (control).
For detailed information on position types and selecting positions, see Position Types on page 75 and
Selected Media Type on page 72.
Note: Wash positions contain wash solution. Reagent positions contain reagent that can be
added to other positions in the plate map.
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