Probe Cleaning; Loader Movement - BIO RAD ZE5 User Manual

Cell analyzer and everest software
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Probe Cleaning

The integrated sample line wash station is located at the bottom of the gauge. The wash station can
introduce either sheath fluid (DI water with additive) or cleaning fluid (from the cleaner bottle) to the
sample line, depending on the process and instrument state.
You can program washes into the sample run during run list setup, and you can specify both the
inside and outside wash times. These washes use filtered (0.2 µm) sheath fluid. The outside of the
sample probe is cleaned by moving the contaminated portion of the sample probe up and down
through the wash station. The inside of the sample line is cleaned by moving the probe up and down
through the wash station while the sample pump is running. This method introduces air and sheath
segments into the sample line and pushes residual sample and debris off the walls and through to
waste. This results in highly effective cleaning and minimal carryover between samples.
The shutdown process uses cleaning fluid to clean the probe and sample line. For more information,
see
Shutting Down on page

Loader Movement

To sample from various types of media, the loader and probe must move in three directions:
n
Left-right (x-axis)
n
In and out (y-axis)
n
Up and down (z-axis)
161.
Probe Cleaning
User Guide | 53

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